Anti-Ghrelin (human)

Anti-Ghrelin (human)

Cat. No.

ABS 050-45

Product name

Anti-Ghrelin (human)

Description

Mouse monoclonal antibody

Specificity

ABS 050-45 binds human octanoylated ghrelin and human des-acyl ghrelin.
 

Immunogen

Synthetic human Ser3-octanoyl ghrelin-28

Application

ELISA, WB

Species reactivity (positive)

Human, rat (5%)

Species reactivity (negative)

Not determined

Subclass

IgG1/l

Gene ID

51738

Clone number

45

Epitope

Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin.