pLKO.3G

pLKO.3G

载体基本信息

出品公司:	Ybscience
载体名称:	pLKO.3G
质粒类型:	慢病毒系统载体；RNAi
高拷贝/低拷贝:	高拷贝
启动子:	U6
克隆方法:	多克隆位点，限制性内切酶
载体大小:	8174bp
5' 测序引物及序列:	GACTATCATATGCTTACCGT
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	Ampicillian
筛选标记:	eGFP
备注:	克隆菌株Stbl3
稳定性:	--
组成型:	--
病毒/非病毒:	慢病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pLKO.3G	8174 bp 	DNA	SYN
DEFINITION  pLKO.3G
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="pLKO.3G"
                     /mol_type="other DNA"
     misc_feature    168..183
                     /label="cPPT"
     CDS             693..1652
                     /label="ORF frame 3"

     gene            936..1649
                     /label="EGFP"
                     /gene="EGFP"

     misc_feature    complement(978..999)
                     /label="EGFP_N_primer"

     misc_feature    1116..1145
                     /label="Y66 (EGFP)"

     misc_feature    1586..1607
                     /label="EGFP_C_primer"

     misc_feature    1673..2260
                     /label="WPRE"

     CDS             1761..2312
                     /label="ORF frame 3"

     CDS             1774..2544
                     /label="ORF frame 1"

     misc_feature    complement(2263..2279)
                     /label="pBluescriptKS_primer"

     misc_feature    2448..2469
                     /label="U3PPT"

     misc_feature    2448..2463
                     /label="cPPT"

     misc_feature    2465..2517
                     /label="delta_U3"

     misc_feature    2518..2698
                     /label="HIV-1_5_LTR"

     misc_feature    2518..2698
                     /label="truncHIV-1_3_LTR"

     terminator      2773..2892
                     /label="SV40_PA_terminator"

     misc_feature    2861..2880
                     /label="EBV_rev_primer"

     rep_origin      2910..3008
                     /label="SV40_origin"

     misc_feature    2993..3012
                     /label="SV40pro_F_primer"

     promoter        complement(3087..3105)
                     /label="T7_promoter"

     promoter        complement(3115..3131)
                     /label="M13_forward20_primer"

     misc_feature    complement(3124..3146)
                     /label="M13_pUC_fwd_primer"

     misc_feature    3124..3272
                     /label="lacZ_a"

     rep_origin      3290..3596
                     /label="f1_origin"

     promoter        3789..3817
                     /label="AmpR_promoter"

     gene            3859..4719
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             3859..4719
                     /label="ORF frame 1"

     rep_origin      4874..5493
                     /label="pBR322_origin"

     promoter        5802..5831
                     /label="lac_promoter"

     misc_feature    5845..5867
                     /label="M13_pUC_rev_primer"

     promoter        5866..5884
                     /label="M13_reverse_primer"

     promoter        5901..5920
                     /label="T3_promoter"

     misc_feature    6174..6354
                     /label="HIV-1_5_LTR"

     misc_feature    6174..6354
                     /label="truncHIV-1_3_LTR"

     misc_feature    6465..6509
                     /label="HIV-1_psi_pack"

     CDS             6897..7754
                     /label="ORF frame 3"

     misc_feature    7019..7252
                     /label="RRE"

     promoter        7983..65
                     /label="hU6_promoter"

     misc_feature    8169..14
                     /label="LKO1_5_primer"

ORIGIN
    1 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
   61 ggacgaaaca ccggtccgca ggtatgcttg cgtgaattcc acctcgagct acttaattaa
  121 accagttttc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
  181 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
  241 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
  301 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
  361 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
  421 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
  481 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
  541 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
  601 gacggacagc gccagggagc aatggcagcg cgccgaccgc ggaagccgca cgtctcacta
  661 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
  721 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
  781 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
  841 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
  901 tctctcccca gggggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca
  961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
 1021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
 1081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
 1141 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
 1201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
 1261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
 1321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
 1381 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
 1441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
 1501 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
 1561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
 1621 tcactctcgg catggacgag ctgtacaagt aagatatcaa gcttatcgat aatcaacctc
 1681 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
 1741 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
 1801 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
 1861 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
 1921 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
 1981 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
 2041 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
 2101 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
 2161 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
 2221 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
 2281 aacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
 2341 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
 2401 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
 2461 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
 2521 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
 2581 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
 2641 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
 2701 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
 2761 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
 2821 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
 2881 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
 2941 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
 3001 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
 3061 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
 3121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
 3181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
 3241 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
 3301 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
 3361 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
 3421 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
 3481 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
 3541 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
 3601 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
 3661 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
 3721 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
 3781 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
 3841 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
 3901 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
 3961 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
 4021 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
 4081 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
 4141 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
 4201 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
 4261 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
 4321 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
 4381 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
 4441 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
 4501 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
 4561 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
 4621 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
 4681 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
 4741 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
 4801 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
 4861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
 4921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
 4981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
 5041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
 5101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
 5161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
 5221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
 5281 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
 5341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
 5401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
 5461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
 5521 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
 5581 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
 5641 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
 5701 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
 5761 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
 5821 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
 5881 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
 5941 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
 6001 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
 6061 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
 6121 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
 6181 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
 6241 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
 6301 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
 6361 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
 6421 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
 6481 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
 6541 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
 6601 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
 6661 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
 6721 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
 6781 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
 6841 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
 6901 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
 6961 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
 7021 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
 7081 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
 7141 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
 7201 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
 7261 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
 7321 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
 7381 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
 7441 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
 7501 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
 7561 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
 7621 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
 7681 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
 7741 gatcacgaga ctagcctcga ccacgacgca atggagaaag acggagagcg ccaacggcgt
 7801 ccatctcgaa ggagtcgcca gcgataaccg gagtagttga aatggtaata agacgaccaa
 7861 tctgaccagc aaggaagcca agatgggaaa ggtcatgcgg catacgctcg gcgccagttt
 7921 gaatattaga cataatttat cctcaagtaa gggggtacac gcgtcagtcg agtcctgtcg
 7981 acaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
 8041 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
 8101 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
 8161 ttaaaatgga ctat