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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pCDH-CMV-MCS-EF1-copGFP
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pCDH-CMV-MCS-EF1-copGFP
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pCDH-CMV-MCS-EF1-copGFP |
| 质粒类型: | 慢病毒系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7544bp |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillian） |
| 筛选标记: | copGFP |
| 备注: | 克隆菌株Stbl3；包装质粒pPACKH1 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
此序列来自于测序结果
LOCUS pCDH-CMV-MCS-EF1-copGFP 7541 bp ds-DNA circular SYN 25-JUL-2016
DEFINITION pCDH-CMV-MCS-EF1-copGFP.
SOURCE http://www.biofeng.com
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7541)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, July 25, 2016 from SnapGene Viewer 3.1.4 to Vector
NTI(R) format
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|776304000|
COMMENT VNTDBDATE|776304000|
COMMENT VNTNAME|pCDH-CMV-MCS-EF1-copGFP|
FEATURES Location/Qualifiers
misc_feature 7..234
/label=RSV
misc_feature 235..414
/label=5'LTR
/note="HIV-1 5'LTR"
misc_feature 522..566
/label=HIV-1_psi_pack
misc_feature 567..919
/label=Gag
CDS 954..1454
/codon_start=1
/label=ORF frame 3
misc_feature 1076..1308
/label=RRE
CDS 1309..1797
/codon_start=1
/label=Env
misc_feature 1798..1916
/label=cPPT
promoter 1922..2271
/label=CMV promoter
primer_bind 2164..2184
/label=CMV_fwd_primer
primer_bind 2208..2227
/label=pCEP_fwd_primer
primer_bind 2210..2234
/label=LNCX_primer
misc_feature 2278..2320
/label=MCS
promoter 2315..2860
/label=EF1-alpha promoter
/note="EF1-alpha promoter"
misc_feature 2875..3629
/label=copGFP
misc_feature 3640..4229
/label=WPRE
CDS 3726..4271
/codon_start=1
/label=ORF frame 2
CDS 3739..4329
/codon_start=1
/label=ORF frame 3
misc_feature 4291..4312
/label=U3PPT
misc_feature 4291..4306
/label=cPPT
misc_feature 4301..4534
/label=3-Delta-LTR
/note="truncHIV-1 3-Delta-LTR"
misc_feature 4308..4360
/label=Delta-U3
/note="Delta-U3"
misc_feature 4361..4541
/label=HIV-1 5'LTR
terminator 4616..4747
/label=SV40 PA
/note="SV40 PA terminator"
misc_feature 4704..4723
/label=EBV_rev_primer
rep_origin 4746..4892
/direction=RIGHT
/label=SV40 ori
/note="SV40 origin"
misc_feature 4819..4834
/label=SV40pro_F_primer
promoter complement(4922..4940)
/label=M13_reverse_primer promoter
misc_feature 4939..4961
/label=M13_pUC_rev_primer
promoter complement(4975..5004)
/label=lac promoter
repeat_region complement(5262..5935)
/label=pUC Ori
/note="complementary chain"
misc_feature complement(6080..6940)
/gene="Ampicillin"
/label=Ampicillin
CDS complement(6087..6947)
/codon_start=1
/label=ORF frame 2
promoter complement(6989..7017)
/label=AmpR promoter
misc_feature 7176..7198
/label=pGEX_3_primer
misc_feature complement(7397..7540)
/label=lacZ a
misc_feature 7511..7533
/label=M13_pUC_fwd_primer
promoter 7526..7541
/label=M13_forward20_primer promoter
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
2281 agagctagcg aattcgaatt taaatcggat ccgcggccgc gaaggatctg cgatcgctcc
2341 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
2401 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
2461 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
2521 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
2581 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
2641 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
2701 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
2761 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
2821 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatggagag
2881 cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg
2941 cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa
3001 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat
3061 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca
3121 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt
3181 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt
3241 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa
3301 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg
3361 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt
3421 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg
3481 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa
3541 gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac
3601 cgccggaccc ggctccaccg gatctcgctg tcgacaatca acctctggat tacaaaattt
3661 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
3721 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
3781 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
3841 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
3901 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
3961 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
4021 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
4081 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
4141 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
4201 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
4261 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
4321 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
4381 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
4441 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
4501 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
4561 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
4621 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
4681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
4741 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
4801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
4861 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
4921 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
4981 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
5041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
5101 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
5161 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
5221 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
5281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
5341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
5401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
5461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
5521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
5581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
5641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
5701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
5761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
5821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
5881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
5941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
6001 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
6061 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
6121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
6181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
6241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
6301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
6361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
6421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
6481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
6541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
6601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
6661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
6721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
6781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
6841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
6901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
6961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
7021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
7081 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
7141 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
7201 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
7261 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
7321 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
7381 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
7441 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
7501 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
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pCDH-CMV-MCS-EF1-copGFP
¥800 - 1200
