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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pCambia1390
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pCambia1390
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pCAMBIA1390 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 8861 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6xHis |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCambia1390 8860 bp DNA circular SYN 24-APR-2000
DEFINITION Binary vector pCAMBIA-1390, complete sequence.
ACCESSION AF234307
VERSION AF234307.1 GI:7638112
KEYWORDS .
SOURCE Binary vector pCAMBIA-1390
ORGANISM Binary vector pCAMBIA-1390
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
REFERENCE 1 (sites)
AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors
for plant transformation
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
PUBMED 7919218
REFERENCE 2 (bases 1 to 8860)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE A comprehensive set of modular vectors for advanced manipulations
and efficient transformation of plants
JOURNAL Unpublished
REMARK Full description of constructs
REFERENCE 3 (bases 1 to 8860)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES Location/Qualifiers
source 1..8860
/organism="Binary vector pCAMBIA-1390"
/mol_type="other DNA"
/db_xref="taxon:118399"
misc_feature 61..313
/note="nos (nopaline synthase) 3'UTR (polyA signal)"
misc_feature 351..376
/note="right border T-DNA repeat"
misc_feature complement(1417..2417)
/note="STA region from pVS1 plasmid"
rep_origin complement(3010..4010)
/note="pVS1-REP; replication origin from pVS1"
misc_feature complement(4420..4680)
/note="bom site from pBR322"
rep_origin complement(4820..5100)
/note="pBR322 origin of replication"
CDS complement(5391..6185)
/note="aadA (kanamycin resistance) gene amplified from
pIG121Hm"
/codon_start=1
/product="aminoglycoside phosphotransferase"
/protein_id="AAF65373.1"
/db_xref="GI:7638113"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6610..6635
/note="left border repeat from C58 T-DNA"
misc_feature 6702..6910
/note="CaMV 3'UTR (polyA signal)"
CDS complement(6926..7951)
/note="hptII (hygromycin resistance) gene"
/codon_start=1
/product="hygromycin phosphotransferase"
/protein_id="AAF65374.1"
/db_xref="GI:7638114"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKK"
promoter complement(7987..8767)
/note="CaMV35S2; CaMV 35S promoter, duplicated"
misc_feature complement(8785..8820)
/note="pUC9 MCS; polylinker"
ORIGIN
1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
841 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
901 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
961 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
1021 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
1081 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
1141 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
1201 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
1261 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
1321 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
1381 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
1441 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
1501 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
1561 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
1621 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
1681 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
1741 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
1801 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
1861 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
1921 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
1981 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
2041 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
2101 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
2161 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
2221 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
2281 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
2341 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
2401 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
2461 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
2521 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
2581 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
2641 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
2701 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
2761 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
2821 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
2881 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
2941 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
3001 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
3061 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
3121 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
3181 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
3241 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
3301 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
3361 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
3421 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
3481 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
3541 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
3601 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
3661 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
3721 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
3781 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
3841 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
3901 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
3961 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
4021 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
4081 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
4141 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
4201 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
4261 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
4321 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
4381 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
4441 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
4501 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
4561 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
4621 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
4681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
4741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
4801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
4861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
4921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
4981 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
5041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
5101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
5161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
5221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
5281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
5341 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
5401 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
5461 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
5521 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
5581 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
5641 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
5701 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
5761 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
5821 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
5881 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
5941 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
6001 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
6061 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
6121 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
6181 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
6241 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
6301 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
6361 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
6421 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
6481 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
6541 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
6601 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
6661 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
6721 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
6781 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
6841 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
6901 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
6961 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
7021 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
7081 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
7141 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
7201 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
7261 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
7321 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
7381 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
7441 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
7501 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
7561 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
7621 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
7681 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
7741 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
7801 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
7861 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
7921 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag
7981 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
8041 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc
8101 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
8161 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
8221 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
8281 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
8341 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
8401 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt
8461 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
8521 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
8581 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
8641 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
8701 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
8761 ccaccatgtt gggcccggcg cgccaagctt ggctgcaggt cgacggatcc ccgggaattc
8821 taagaggagt ccaccatggt agatctgact agtgttaacg
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文献和实验相关实验
在 blood 杂志上，通讯作者是美国明尼苏达大学医学院的 Verfaillie CM 教授，由于文章中多个数据重复使用，作者主动要求撤回，2009 年该论文遭撤稿，被引用 1698 次。4 被引用 1582 次这篇文章是英国约翰英纳斯中心的 Baulcombe D 教授于 2003 年发表在 PLANTJOURNAL 杂志上的文章，2015 年被撤回，目前被引用 1582 次撤稿原因是 WB 图片重复利用。5 被引用 1390 次该文于 2003 年发表在医学顶级杂志 Lancet 杂志上，2009 年
-DNA or transposon) insertions using the TAIL-PCR method. The amplified products are suitable as templates for direct sequencing, or for cloning in vectors. Examples are given in use of the protocol for T-DNA tagging in rice using the pCAMBIA binary vectors.
plants, bypassing the callus formation phase. Stolon nodes are infected and co-cultivated with Agrobacterium tumefaciens harboring pCAMBIA binary vectors. Hygromycin phosphotransferase gene (hph ) is used as the selectable marker and hygromycin is used
技术资料暂无技术资料 索取技术资料
pCambia1390
¥800 - 1200
