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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
6个月
- 英文名:
pFastBacI-Gus
- 库存:
大量
- 供应商:
钰博生物
- 规格:
1ug/5ug
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pFastBacI-Gus |
| 质粒类型: | 昆虫细胞表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | 6661bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Ampicillin |
| 筛选标记: | Gentamicin |
| 备注: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 杆状病毒 |
载体质粒图谱和多克隆位点信息


载体简介
pFastBac1-Gus is a 6661 bp control vector containing the Arabidopsis thaliana gene for β-glucuronidase (Gus) (Kertbundit et al., 1991), and was generated by restriction cloning of the Gus gene into pFastBac1. The molecular weight of β-glucuronidase is 68.5 kDa.
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文献和实验Patterns in the localization of compounds, metabolic processes, and regulatory machineries at the cellular and subcellular levels are studied by scientific disciplines, called cytochemistry (biochemistry of the cell, cellular topochemistry
(Janine’s protocol) 1. Add 200µl of acetone, vortex briefly. 2. Remove acetone and pipette into flat-bottom 96-well plate (polystyrene) 3. Centrifuge plate at 3000 x g for 3-5 minutes. 4. Invert plate carefully and blot dry
一、实验试剂 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g Triton X-100 0.5 g N-Lauroylsarcosine Sodium Salt (=0.1%) Extraction Buffer (500ul
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