pCDH-EF1-MCS-T2A-copGFP

pCDH-EF1-MCS-T2A-copGFP

载体基本信息

出品公司:	Ybscience
载体名称:	pCDH-EF1-MCS-T2A-copGFP
质粒类型:	慢病毒表达载体；cDNA克隆载体；荧光报告载体
克隆方法:	多克隆位点，限制性内切酶
启动子:	EF1α
载体大小:	--
5' 测序引物及序列:	5’-CTCCACGCTTTGCCTGACCCTGCTT-3’
3' 测序引物及序列:	--
载体标签:	无
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	GFP
克隆菌株:	stbl3
宿主细胞（系）:	大部分细胞类型（分裂与非分裂细胞）；初级分化细胞
备注:	pCDH-EF1-MCS-T2A-copGFP慢病毒表达载体是基于HIV的慢病毒载体； 用于cDNA表达和克隆；高效转染细胞，建立稳定细胞系； 单一启动子，由EF1a启动子驱动目的基因和与荧光报告基因转录为双顺反子； 二者之间含一段T2A肽，能够在翻译后水平上自我剪切，保证目的蛋白与荧光蛋白同等水平翻译。
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	慢病毒（HIV)

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体介绍：
Stable overexpression with cDNA lentivectors
Strong and ubiquitous expression of the gene of interest
Single or double expression cassette with choice of reporter gene
Target gene expressed from CMV, EF1, or MSCV promoter
Choose from FIV- or HIV-based vectors
Gene expression from constitutive promoters
The multiple cloning site (MCS) located downstream of an internal promoter allows convenient cloning of your gene of interest.


优点：
Reliable delivery to dividing or non-dividing cells
When used with lentiviral packaging plasmids, your cDNA constructs can be packaged into VSV-G pseudotyped viral particles and delivered into a wide range of mammalian cells with high efficiency. Stable cell lines can be generated that express the gene of interest. The system can also be used to overexpress genes in model organisms.

Convenient selection of transduced cells
In addition to the pCD-MCS one-promoter vectors, which feature the Multiple Cloning Site (MCS) downstream of the CMV promoter, the pCD system offers the option of a second expression cassette downstream of the MCS to express the puromycin resistance gene or copGFP as a reporter under the control of a constitutive human elongation factor 1α (EF1) promoter, which is functional in most cell lines.

Efficient coexpression of target and reporter with T2A peptide
SBI’s most recent additions to the cDNA vector collection confront the problems of promoter interference and imbalanced expression by incorporating a “self-cleaving” T2A peptide derived from the insect virus Thosea asigna to mediate coexpression of a reporter gene with the target cDNA. This allows an easy way to track cells actively expressing your gene of interest.

The CD52X EF1 or CMV Promoter Series with T2A Co-expressed markers
SBI’s cDNA expression vectors using the EF1 promoter (CD520A-1, CD521A-1) or CMV (CD524A-1) driving expression also incorporates the 2A-like sequence (T2A) from the insect virus Thosea asigna to mediate the co-expression of a reporter gene with the target cDNA. Reporter genes have been cloned at either the first or second positions relative to the T2A element, and both markers achieved high expression levels at either position.