pMT-Bip-V5-HisA

pMT-Bip-V5-HisA

载体基本信息

出品公司:	Ybscience
载体名称:	pMT-Bip-V5-HisA
质粒类型:	昆虫细胞表达载体
高拷贝/低拷贝:	--
启动子:	P-MT (Metallothionein)
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3642bp
5' 测序引物及序列:	Metallothionein: 5'd[CATCTCAGTGCAACTAAA]3'
3' 测序引物及序列:	--
载体标签:	BiP (N), V5 (C), His (C)
载体抗性:	Ampicillin
筛选标记:	--
备注:	使用硫酸铜或氯化镉作为诱导剂。
稳定性:	--
组成型:	诱导型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

 

载体序列

LOCUS       pMT/BIP/V5-HisA           3642 bp ds-DNA    circular SYN 09-四月-2014
DEFINITION  Drosophila vector for inducible high-level expression of secreted 
            and tagged proteins. For other reading frames, use pMT/BiP/V5-His B 
            or pMT/BiP/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pMT BiP V5-His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     To create stable cell lines, co-transfect with the drug selection 
            vector pCoHygro or pCoBlast.
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     promoter        411..833
                     /label="MT promoter"
                     /label="Drosophila metallothionein promoter"
     CDS             851..904
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label="BiP signal sequence"
                     /translation="MKLCILLAVVAFVGLSLG"
     misc_feature    905..1002
                     /label="MCS"
                     /label="multiple cloning site"
     CDS             1003..1044
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label="V5 tag"
                     /translation="GKPIPNPLLGLDST"
     CDS             1054..1071
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label="6xHis"
                     /translation="HHHHHH"
     polyA_signal    1144..1278
                     /label="SV40 poly(A) signal"
                     /label="SV40 polyadenylation signal"
     rep_origin      complement(1824..2412)
                     /direction=LEFT
                     /label="ori"
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2583..3443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label="AmpR"
                     /label="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(3444..3548)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattttaa cgttgcagga
      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata
      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc
      841 cgatctcaat atgaagttat gcatattact ggccgtcgtg gcctttgttg gcctctcgct
      901 cgggagatct ccatggcccg gggtacctac tagtccagtg tggtggaatt ctgcagatat
      961 ccagcacagt ggcggccgct cgagtctaga gggcccttcg aaggtaagcc tatccctaac
     1021 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
     1081 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc
     1141 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     1201 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     1261 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     1321 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     1381 gatcagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1441 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1501 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1561 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2581 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2641 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2701 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2761 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2821 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2881 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2941 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3001 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3061 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3121 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3181 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3241 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3301 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3361 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3421 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3481 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3541 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3601 acattaacct ataaaaatag gcgtatcacg aggccctttc gt