pRS426

pRS426

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  • Ybscience
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  • 2025年07月11日
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃

    • 保质期：

      6个月

    • 英文名：

      pRS426

    • 库存：

      大量

    • 供应商：

      钰博生物

    • 规格：

      1ug/5ug

    pRS426

    载体基本信息

    出品公司: Ybscience
    载体名称: pRS426
    质粒类型: 酿酒酵母表达载体
    高拷贝/低拷贝: --
    启动子: --
    克隆方法: 多克隆位点，限制性内切酶
    载体大小: 5726bp
    5' 测序引物及序列: --
    3' 测序引物及序列: --
    载体标签: --
    载体抗性: Ampicillin
    筛选标记: --
    备注: --
    稳定性: --
    组成型: --
    病毒/非病毒: 非病毒
     

    载体质粒图谱和多克隆位点信息

    pRS426载体图谱

    载体简介

     

    The order of the major features in this plasmid is:ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori (2 micron) - URA3. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 + or - 1.4% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PstI--4.0, 1.7; PvuII--5.2, 0.54. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416322 Hosts: E.coli DH5alpha, Saccharomyces cerevisiae, E.coli. Related vectors: pRS424, pRS425, pRS423. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)

     

    载体序列

    LOCUS       pRS426	5726 bp 	DNA	SYN
DEFINITION  pRS426
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="pRS426"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     promoter        202..415
                     /label="URA3_promoter"
     gene            417..1217
                     /label="URA3"
                     /gene="URA3"
     CDS             417..1220
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             complement(452..928)
                     /label="ORF frame 2"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             complement(1589..2167)
                     /label="ORF frame 2"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(1822..1965)
                     /label="lacZ_a"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    1936..1958
                     /label="M13_pUC_fwd_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        1951..1967
                     /label="M13_forward20_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        1977..1995
                     /label="T7_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    2020..2036
                     /label="pBluescriptKS_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(2071..2087)
                     /label="pBluescriptSK_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(2123..2142)
                     /label="T3_promoter"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     promoter        complement(2159..2177)
                     /label="M13_reverse_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     misc_feature    complement(2176..2198)
                     /label="M13_pUC_rev_primer"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     gene            complement(3324..4184)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS
                     IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE
                     ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA
                     TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
     CDS             complement(3324..4184)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4226..4254)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(5279..5326)
                     /label="FRT"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
  241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
  301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
  361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
  421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
  481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
  541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
  601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
  661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
  721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
  781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
  841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
  901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
  961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
 1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
 1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
 1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
 1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
 1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
 1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
 1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
 1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
 1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
 1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
 1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
 1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
 1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
 1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
 1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
 1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
 1981 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
 2041 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
 2101 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
 2161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga
 2221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt
 2281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
 2341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
 2401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
 2461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
 2521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
 2581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
 2641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
 2701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
 2761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
 2821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
 2881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
 2941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
 3001 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
 3061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
 3121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
 3181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
 3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
 3301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
 3361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
 3421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
 3481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
 3541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
 3601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
 3661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
 3721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
 3781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
 3841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
 3901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
 3961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
 4021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
 4081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
 4141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
 4201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
 4261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaacga
 4321 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
 4381 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
 4441 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
 4501 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
 4561 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
 4621 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
 4681 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
 4741 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
 4801 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
 4861 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
 4921 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
 4981 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
 5041 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
 5101 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
 5161 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
 5221 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
 5281 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
 5341 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
 5401 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
 5461 ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
 5521 ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
 5581 tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
 5641 ttcctttgat attggatcat actaagaaac cattattatc atgacattaa cctataaaaa
 5701 taggcgtatc acgaggccct ttcgtc

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