pCMV-Red-Firefly-Luc

pCMV-Red-Firefly-Luc

载体基本信息

出品公司:	Ybscience
载体名称:	pCMV-Red-Firefly-Luc ; pCMV-Red Firefly Luc
质粒类型:	哺乳动物载体；红色荧光素酶报告载体
高拷贝/低拷贝:	--
克隆方法:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	6575 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	Red Firefly Luciferase红色萤火虫荧光素酶
载体抗性:	氨苄青霉素
筛选标记:	嘌呤霉素（Puromycin）
克隆菌株:	TOP10等常规菌株
宿主细胞（系）:	常规哺乳动物细胞
备注:	--
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

pCMV-Red Firefly Luc载体说明

Thermo Scientific pCMV-Red Firefly Luc is a constitutive expression vector having the luciferase gene under the CMV (Cytomegalovirus) promoter for co-transfection and normalization control to account for experimental variation in combination with other reporters in a gene regulation study using the intracellular red firefly luciferase reporter with excellent light intensity..

Features of Red Firefly Luc:
• Red Firefly Luc Vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.
• pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
• pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively.
• These pCMV and pTK constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

These vectors are subject to a limited use label license.

More Product Data
• Highly sensitive multiplex luciferase reporter assays
• Monitoring neuronal differentiation using multiplexed luciferase reporters
• Activation of the antioxidant response pathway by pesticide chemicals

 

载体序列

LOCUS       pCMV-Red Firefly Luc	6575 bp 	DNA	SYN
DEFINITION  pCMV-Red Firefly Luc
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="pCMV-Red Firefly Luc"
                     /mol_type="other DNA"
     promoter        13..605
                     /label="CMV_immearly_promoter"
     misc_feature    68..355
                     /label="CAG_enhancer"
     misc_feature    522..542
                     /label="CMV_fwd_primer"
     CDS             527..2293
                     /label="ORF frame 2"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     promoter        616..634
                     /label="T7_promoter"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     misc_feature    complement(2313..2330)
                     /label="BGH_rev_primer"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     misc_feature    complement(2548..2568)
                     /label="pBABE_3_primer"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     misc_feature    complement(2554..2769)
                     /label="SV40_enhancer"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     promoter        2566..2834
                     /label="SV40_promoter"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     rep_origin      2733..2810
                     /label="SV40_origin"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     misc_feature    2795..2814
                     /label="SV40pro_F_primer"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     promoter        2928..2995
                     /label="EM7_promoter"
                     /translation="MGGRRVRWEVYISRALWLTREPTAYWLIEINTTHYRGSATMENM
                     ENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFTNAVTGVDYSYAEYLEKS
                     CCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYTLRELVHSL
                     GISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGYQCLDTFIKRNTPPGF
                     QASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPG
                     TAVLTVVPFHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQDYKCTYVILVPTLF
                     AILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQGYGLTETTSAII
                     ITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEAT
                     KELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFD
                     AGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPK
                     GLTGKIDGRAIREILKKPVAKM*"
     CDS             2996..3595
                     /label="ORF frame 2"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    3816..3835
                     /label="EBV_rev_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     gene            complement(3899..4759)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     CDS             complement(3899..4759)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4801..4829)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5007..5626
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5881..5897
                     /label="M13_forward20_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6397..605
                     /label="CMV_immearly_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6524..6543
                     /label="RVprimer3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 AACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
   61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
  121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
  181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
  241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
  301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
  361 TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG
  421 GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA
  481 ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG
  541 TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA
  601 CTGGCTTATC GAAATTAATA CGACTCACTA TAGGGGATCC GCCACCATGG AAAATATGGA
  661 AAACGACGAG AACATCGTGG TGGGCCCCAA GCCCTTCTAC CCCATCGAGG AAGGCAGCGC
  721 CGGCACCCAG CTGCGGAAGT ACATGGAAAG ATACGCCAAG CTGGGCGCCA TTGCCTTCAC
  781 CAACGCCGTG ACCGGCGTGG ACTACAGCTA CGCCGAGTAC CTGGAAAAGA GCTGCTGCCT
  841 GGGCAAGGCT CTGCAGAACT ACGGCCTGGT GGTGGACGGC CGGATCGCCC TGTGCAGCGA
  901 GAACTGCGAG GAATTCTTCA TCCCCGTGAT CGCCGGCCTG TTCATCGGCG TGGGCGTGGC
  961 TCCCACCAAC GAGATCTACA CCCTGCGGGA GCTGGTGCAC AGCCTGGGCA TCAGCAAGCC
 1021 CACCATCGTG TTCAGCAGCA AGAAGGGCCT GGACAAAGTC ATCACCGTGC AGAAAACCGT
 1081 GACCACCATC AAGACCATCG TGATCCTGGA CAGCAAGGTG GACTACCGGG GCTACCAGTG
 1141 CCTGGACACC TTCATCAAGC GGAACACCCC CCCTGGCTTC CAGGCCAGCA GCTTCAAGAC
 1201 CGTGGAGGTG GACCGGAAAG AACAGGTGGC CCTGATCATG AACAGCAGCG GCAGCACCGG
 1261 CCTGCCCAAG GGCGTGCAGC TGACCCACGA GAACACCGTG ACCCGGTTCA GCCACGCCAG
 1321 GGACCCCATC TACGGCAACC AGGTGTCCCC CGGCACCGCC GTGCTGACCG TGGTGCCCTT
 1381 CCACCACGGC TTCGGCATGT TCACCACCCT GGGCTACCTG ATCTGCGGCT TCCGGGTGGT
 1441 GATGCTGACC AAGTTCGACG AGGAAACCTT CCTGAAAACC CTGCAGGACT ACAAGTGCAC
 1501 CTACGTGATT CTGGTGCCCA CCCTGTTCGC CATCCTGAAC AAGAGCGAGC TGCTGAACAA
 1561 GTACGACCTG AGCAACCTGG TGGAGATCGC CAGCGGCGGA GCCCCCCTGA GCAAAGAAGT
 1621 GGGAGAGGCC GTCGCCAGGC GGTTCAATCT GCCCGGCGTG CGGCAGGGCT ACGGCCTGAC
 1681 CGAGACAACC AGCGCCATCA TCATCACCCC CGAGGGCGAC GACAAGCCTG GAGCCAGCGG
 1741 CAAGGTGGTG CCCCTGTTCA AGGCCAAAGT GATCGACCTG GACACCAAGA AGAGCCTGGG
 1801 CCCCAACAGA CGGGGCGAAG TGTGCGTGAA GGGCCCCATG CTGATGAAGG GCTACGTGAA
 1861 CAACCCCGAG GCCACCAAAG AGCTGATCGA CGAAGAGGGC TGGCTGCACA CCGGCGACAT
 1921 CGGCTACTAC GACGAAGAGA AGCACTTCTT CATCGTGGAC CGGCTGAAGA GCCTGATCAA
 1981 GTACAAGGGC TATCAGGTGC CCCCTGCCGA GCTGGAAAGC GTCCTGCTGC AGCACCCCAG
 2041 CATCTTCGAC GCCGGCGTGG CCGGGGTGCC AGATCCTGTG GCCGGCGAGC TGCCTGGCGC
 2101 CGTGGTGGTG CTGGAATCCG GCAAGAACAT GACCGAGAAA GAAGTGATGG ACTACGTCGC
 2161 CAGCCAGGTG TCCAACGCCA AGCGGCTGAG AGGCGGCGTG AGATTCGTGG ACGAAGTGCC
 2221 AAAGGGCCTG ACCGGCAAGA TCGACGGCAG GGCCATCCGG GAGATCCTGA AGAAACCCGT
 2281 GGCCAAGATG TGATGAGCGG CCGCAAAATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC
 2341 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT
 2401 CCTTTCCTAA TAAAATGAGG AAATTGCATC ACAACACTCA ACCCTATCTC GGTCTATTCT
 2461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA
 2521 CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC
 2581 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT
 2641 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT
 2701 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG
 2761 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT
 2821 CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
 2881 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
 2941 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGAC
 3001 CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC
 3061 CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA
 3121 CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG
 3181 CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT
 3241 CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG
 3301 GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC
 3361 GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC
 3421 CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC
 3481 ATCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT
 3541 CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACACGT
 3601 GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT
 3661 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA
 3721 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
 3781 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
 3841 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATT
 3901 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
 3961 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
 4021 GCGCTGCGAT GATACCGCGA GAACCACGCT CACCGGCTCC GGATTTATCA GCAATAAACC
 4081 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
 4141 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
 4201 TTGTTGCCAT CGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
 4261 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
 4321 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
 4381 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
 4441 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
 4501 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
 4561 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
 4621 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
 4681 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
 4741 GGAAATGTTG AATACTCATA TTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
 4801 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCA
 4861 GTGTTACAAC CAATTAACCA ATTCTGAACA TTATCGCGAG CCCATTTATA CCTGAATATG
 4921 GCTCATAACA CCCCTTGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC GCGCGTCGTT
 4981 CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT
 5041 GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC
 5101 GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC
 5161 AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGCCCACCAC TTCAAGAACT CTGTAGCACC
 5221 GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC
 5281 GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG
 5341 AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA
 5401 CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA
 5461 TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC
 5521 CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG
 5581 ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT
 5641 CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT
 5701 GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA
 5761 GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGGCGAGAGT AGGGAACTGC CAGGCATCAA
 5821 ACTAAGCAGA AGGCCCCTGA CGGATGGCCT TTTTGCGTTT CTACAAACTC TTTCTGTGTT
 5881 GTAAAACGAC GGCCAGTCTT AAGCTCGGGC CCCCTGGGCG GTTCTGATAA CGAGTAATCG
 5941 TTAATCCGCA AATAACGTAA AAACCCGCTT CGGCGGGTTT TTTTATGGGG GGAGTTTAGG
 6001 GAAAGAGCAT TTGTCAGAAT ATTTAAGGGC GCCTGTCACT TTGCTTGATA TATGAGAATT
 6061 ATTTAACCTT ATAAATGAGA AAAAAGCAAC GCACTTTAAA TAAGATACGT TGCTTTTTCG
 6121 ATTGATGAAC ACCTATAATT AAACTATTCA TCTATTATTT ATGATTTTTT GTATATACAA
 6181 TATTTCTAGT TTGTTAAAGA GAATTAAGAA AATAAATCTC GAAAATAATA AAGGGAAAAT
 6241 CAGTTTTTGA TATCAAAATT ATACATGTCA ACGATAATAC AAAATATAAT ACAAACTATA
 6301 AGATGTTATC AGTATTTATT ATCATTTAGA ATAAATTTTG TGTCGCCCTT AATTGTGAGC
 6361 GGATAACAAT TACGAGCTTC ATGCACAGTG GCGTTGACAT TGATTATTGA CTAGCATGTT
 6421 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCATGC ATTAGTTATT
 6481 AATAACATAC GCTCTCCATC AAAACAAAAC GAAACAAAAC AAACTAGCAA AATAGGCTGT
 6541 CCCCAGTGCA AGTGCAGGTG CCAGAACATT TCTCT