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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pSV2-DHFR
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pSV2-DHFR
pSV2DHFR载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pSV2DHFR, pSV2-DHFR, ATCC37146, ATCC 37146 |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | SV40 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4900 bp |
| 5' 测序引物: | SV40pro-F: TATTTATGCAGAGGCCGAGG |
| 3' 测序引物: | SV40pA-R: GAAATTTGTGATGCTATTGC |
| 载体标签: | C-DHFR ( mouse DHFR) |
| 载体抗性: | 氨苄 |
| 筛选标记: | MTX (甲氨蝶呤) |
| 备注: | pSV2-DHFR载体含有的是小鼠的DHFR基因，同时该载体是哺乳动物细胞和大肠杆菌穿梭质粒，在DHFR细胞系中，可以通过甲氨蝶呤对转染的细胞进行筛选。 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
pSV2DHFR载体质粒图谱和多克隆位点信息
pSV2DHFR载体简介
pSV2DHFR载体序列
LOCUS pSV2-DHFR 4900 bp DNA circular SYN
DEFINITION pSV2-DHFR
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4900
/organism="pSV2-dhfr"
/mol_type="other DNA"
misc_feature complement(5..25)
/label="pBABE_3_primer"
misc_feature complement(11..226)
/label="SV40_enhancer"
promoter 23..291
/label="SV40_promoter"
rep_origin 190..267
/label="SV40_origin"
misc_feature 252..271
/label="SV40pro_F_primer"
CDS 362..925
/label="mouse DHFR"
misc_feature 1081..1096
/label="SV40_int"
misc_feature 1102..1149
/label="SV40_3_splice"
CDS 1226..1699
/label="ORF frame 2"
misc_feature 1277..1297
/label="SV40 NLS"
misc_feature 1283..1297
/label="NLS"
terminator 1725..1856
/label="SV40_PA_terminator"
CDS complement(1797..2558)
/label="ORF frame 3"
misc_feature 1813..1832
/label="EBV_rev_primer"
promoter 2744..2772
/label="AmpR_promoter"
gene 2814..3674
/label="Ampicillin"
/gene="Ampicillin"
CDS 2814..3674
/label="ORF frame 3"
rep_origin 3829..4448
/label="pBR322_origin"
misc_feature 4845..4867
/label="pGEX_3_primer"
ORIGIN
1 cTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
61 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA
121 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
181 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
241 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
301 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTATCC CCGCTGCCAT
361 CATGGTTCGA CCATTGAACT GCATCGTCGC CGTGTCCCAA GATATGGGGA TTGGCAAGAA
421 CGGAGACCTA CCCTGGCCTC CGCTCAGGAA CGAGTTCAAG TACTTCCAAA GAATGACCAC
481 AACCTCTTCA GTGGAAGGTA AACAGAATCT GGTGATTATG GGTAGGAAAA CCTGGTTCTC
541 CATTCCTGAG AAGAATCGAC CTTTAAAGGA CAGAATTAAT ATAGTTCTCA GTAGAGAACT
601 CAAAGAACCA CCACGAGGAG CTCATTTTCT TGCCAAAAGT TTGGATGATG CCTTAAGACT
661 TATTGAACAA CCGGAATTGG CAAGTAAAGT AGACATGGTT TGGATAGTCG GAGGCAGTTC
721 TGTTTACCAG GAAGCCATGA ATCAACCAGG CCACCTCAGA CTCTTTGTGA CAAGGATCAT
781 GCAGGAATTT GAAAGTGACA CGTTTTTCCC AGAAATTGAT TTGGGGAAAT ATAAACTTCT
841 CCCAGAATAC CCAGGCGTCC TCTCTGAGGT CCAGGAGGAA AAAGGCATCA AGTATAAGTT
901 TGAAGTCTAC GAGAAGAAAG ACTAACAGGA AGATGCTTTC AAGTTCTCTG CTCCCCTCCT
961 AAAGCTATGC ATTTTTATAA GACCATGGGA CTTTTGCTGG CTTTAGATCT TTGTGAAGGA
1021 ACCTTACTTC TGTGGTGTGA CATAATTGGA CAAACTACCT ACAGAGATTT AAAGCTCTAA
1081 GGTAAATATA AAATTTTTAA GTGTATAATG TGTTAAACTA CTGATTCTAA TTGTTTGTGT
1141 ATTTTAGATT CCAACCTATG GAACTGATGA ATGGGAGCAG TGGTGGAATG CCTTTAATGA
1201 GGAAAACCTG TTTTGCTCAG AAGAAATGCC ATCTAGTGAT GATGAGGCTA CTGCTGACTC
1261 TCAACATTCT ACTCCTCCAA AAAAGAAGAG AAAGGTAGAA GACCCCAAGG ACTTTCCTTC
1321 AGAATTGCTA AGTTTTTTGA GTCATGCTGT GTTTAGTAAT AGAACTCTTG CTTGCTTTGC
1381 TATTTACACC ACAAAGGAAA AAGCTGCACT GCTATACAAG AAAATTATGG AAAAATATTC
1441 TGTAACCTTT ATAAGTAGGC ATAACAGTTA TAATCATAAC ATACTGTTTT TTCTTACTCC
1501 ACACAGGCAT AGAGTGTCTG CTATTAATAA CTATGCTCAA AAATTGTGTA CCTTTAGCTT
1561 TTTAATTTGT AAAGGGGTTA ATAAGGAATA TTTGATGTAT AGTGCCTTGA CTAGAGATCA
1621 TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC
1681 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT
1741 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
1801 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCCCCA
1861 GGAAGCTCCT CTGTGTCCTC ATAAACCCTA ACCTCCTCTA CTTGAGAGGA CATTCCAATC
1921 ATAGGCTGCC CATCCACCCT CTGTGTCCTC CTGTTAATTA GGTCACTTAA CAAAAAGGAA
1981 ATTGGGTAGG GGTTTTTCAC AGACCGCTTT CTAAGGGTAA TTTTAAAATA TCTGGGAAGT
2041 CCCTTCCACT GCTGTGTTCC AGAAGTGTTG GTAAACAGCC CACAAATGTC AACAGCAGAA
2101 ACATACAAGC TGTCAGCTTT GCACAAGGGC CCAACACCCT GCTCATCAAG AAGCACTGTG
2161 GTTGCTGTGT TAGTAATGTG CAAAACAGGA GGCACATTTT CCCCACCTGT GTAGGTTCCA
2221 AAATATCTAG TGTTTTCATT TTTACTTGGA TCAGGAACCC AGCACTCCAC TGGATAAGCA
2281 TTATCCTTAT CCAAAACAGC CTTGTGGTCA GTGTTCATCT GCTGACTGTC AACTGTAGCA
2341 TTTTTTGGGG TTACAGTTTG AGCAGGATAT TTGGTCCTGT AGTTTGCTAA CACACCCTGC
2401 AGCTCCAAAG GTTCCCCACC AACAGCAAAA AAATGAAAAT TTGACCCTTG AATGGGTTTT
2461 CCAGCACCAT TTTCATGAGT TTTTTGTGTC CCTGAATGCA AGTTTAACAT AGCAGTTACC
2521 CCAATAACCT CAGTTTTAAC AGTAACAGCT TCCCACATCA AAATATTTCC ACAGGTTAAG
2581 TCCTCATTTA AATTAGGCAA AGGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT
2641 TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG
2701 GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC
2761 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA
2821 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG
2881 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG
2941 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC
3001 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG
3061 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT
3121 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG
3181 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC
3241 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT
3301 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG
3361 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC
3421 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC
3481 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA
3541 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG
3601 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA
3661 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC
3721 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA
3781 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT
3841 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC
3901 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG
3961 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC
4021 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG
4081 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG
4141 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA
4201 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG
4261 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA
4321 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT
4381 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA
4441 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC
4501 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG
4561 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC
4621 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC
4681 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG
4741 TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC
4801 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG
4861 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG
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文献和实验相关实验
Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
Inactivating a specific protein in vivo can yield important information about its function. One strategy previously developed inSaccharomyces cerevisiae by the Varshavsky group involves fusing a degron, derived from mouse dihydrofolate
细胞系等，该细胞有一定自发转化倾向，一般在转染前一天接种细胞，接种密度为2×104 /cm2 ,用含10%胎牛血清的DMEM液，37℃、5%CO2 培养，待细胞占50～70%瓶底面积时，用于转染试验。 (3)DNA-磷酸钙沉淀物的制备 ①将供体细胞DNA和PSV2-neo质粒载体DNA用TE配制成40mg/L的DNA溶液，同时向供体细胞DNA液200μl中加入带基因neo质粒DNA液(20mg/L)220μl和2×HBS 250μl(PSV2-neo为1～2mg/L的使用量)。 ②取500μl上述
Cytogenetic Characterization of Recombinant Cells
; therefore a selectable system is desirable to discriminate cells that have integrated the foreign DNA. Chinese hamster ovary (CHO) cells are most commonly used because of the availability of both a dihydrofolate reductase (DHFR)-deficient mutant cell line (CHO-DUKX
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pSV2-DHFR
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