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- 2025年07月11日
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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pGEX-2T
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pGEX-2T
pGEX2T载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 别名: | pGEX-2T, pGEX2T, pGEX 2T |
| 质粒类型: | 大肠杆菌蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 启动子: | Tac |
| 克隆方法: | 多克隆位点，限制性内切酶, BamHI，SmaI, EcoRI, XbaI, NcoI, SalI, XhoI, SacI, HindIII |
| 载体大小: | 4898 bp |
| 5' 测序引物及序列: | pGEX5': GGGCTGGCAAGCCACGTTTGGTG |
| 3' 测序引物及序列: | pGEX3': CCGGGAGCTGCATGTGTCAGAGG |
| 载体标签: | N-GST |
| 载体抗性: | Ampicillin 氨苄 |
| 备注: | 复制子是pMB1 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 诱导表达 |
| 病毒/非病毒: | 非病毒 |
pGEX2T载体质粒图谱和多克隆位点信息
pGEX2T载体简介
pGEX2T载体序列
LOCUS pGEX-2T 4948 bp DNA circular SYN
DEFINITION pGEX-2T
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4948
/organism="pGEX-2T"
/mol_type="other DNA"
promoter 184..212
/label="tac_promoter"
misc_feature 224..246
/label="M13_pUC_rev_primer"
gene 258..956
/label="GST (variant)"
/gene="GST (variant)"
CDS 258..956
/label="ORF frame 3"
misc_feature complement(998..1020)
/label="pGEX_3_primer"
promoter 1286..1314
/label="AmpR_promoter"
gene 1356..2216
/label="Ampicillin"
/gene="Ampicillin"
CDS 1356..2216
/label="ORF frame 3"
rep_origin 2371..2990
/label="pBR322_origin"
misc_feature 3288..4379
/label="lacI"
CDS 3420..4379
/label="ORF frame 3"
promoter 4428..4457
/label="lac_promoter"
misc_feature 4471..4493
/label="M13_pUC_rev_primer"
promoter 4492..4510
/label="M13_reverse_primer"
CDS 4502..81
/label="ORF frame 2"
misc_feature 4519..4674
/label="lacZ_a"
promoter complement(4522..4538)
/label="M13_forward20_primer"
misc_feature complement(4531..4553)
/label="M13_pUC_fwd_primer"
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gttccgcgtg gatccccggg aattcatcgt gactgactga
961 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
1021 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
1081 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
1141 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
1201 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
1261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
1321 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
1381 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
1441 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
1501 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
1561 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
1621 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
1681 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
1741 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
1801 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
1861 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
1921 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
1981 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
2041 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
2101 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
2161 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
2221 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
2281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
2341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
2401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
2461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
2521 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
2581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
2641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
2701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
2761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
2821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
2881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
2941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
3001 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
3061 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
3121 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
3181 ttacgcatct gtgcggtatt tcacaccgca taaattccga caccatcgaa tggtgcaaaa
3241 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
3301 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
3361 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
3421 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
3481 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
3541 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
3601 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
3661 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
3721 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
3781 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
3841 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
3901 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
3961 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
4021 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
4081 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
4141 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
4201 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
4261 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
4321 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
4381 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
4441 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
4501 tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
4561 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
4621 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
4681 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
4741 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
4801 ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
4861 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
4921 gcgaattatt tttgatggcg ttggaatt
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pGEX-2T
¥800 - 1200
