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- 2025年07月14日
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文献和实验Isolation and Culture of Schwann Cells
Primarily cultured Schwann cells are essential for the investigation of molecular mechanisms regulating proliferation, survival, differentiation, and myelination of Schwann cell and for the development of efficient transplantation
The method involved in the establishment of human adult Schwann cell cultures has steadily evolved over the last 20 yr. Unlike the more straightforward methods used with other species (e.g., rat), simple dissociation of human peripheral nerve
The most widely used method (the Brockes’ method) for preparing primary Schwann cell culture uses neonatal rat sciatic nerves as the primary source of Schwann cells. The procedure is relatively simple and yields a highly purified population
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