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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human recombinant NGAL adsorbed to aluminium hydroxide gel.
- 亚型:
Mouse IgG1
- 形态:
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
H
- 宿主:
Mouse
- 应用范围:
E, WB
- 浓度:
见详细说明信息
- 靶点:
NGAL (15)
- 抗体英文名:
NGAL (15) Antibody
- 抗体名:
NGAL (15)抗体
- 规格:
0.2 mg
NGAL (15) Antibody产品详细介绍:
| 产品名称: | NGAL (15) Antibody |
| 说明书: | http://www.abbiotec.com/antibodies/ngal-2c10-antibody【参考图片】 |
| 货号: | 250387 |
| 规格: | 0.2 mg |
| 产品描述: | Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury. This antibody is specific for human NGAL. No cross-reaction with NGAL from mouse, rat, pig and dog has been detected. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG1 |
| 免疫原: | Human recombinant NGAL adsorbed to aluminium hydroxide gel. |
| 适用物种: | H |
| 应用范围: | E, WB |
| 应用说明: | Product Cat. No. 250387 reacts in ELISA with human NGAL in solution or coated directly onto the well. Cat. No. 250387 (as capture antibody) forms a sandwich ELISA pair with Products Cat. No. 250386 and 254530 (as biotinylated detection antibody) for measuring human NGAL. When 250387 is used as biotinylated detection antibody with 254530 as capture antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis. E: 1:200; WB: 1:50 |
| 别名: | Neutrophil gelatinase-associated lipocalin; ; 25; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3 |
| 形态: | Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件: | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验人Lipocalin-2 (NGAL)ELISA试剂盒 说明书
2. 以标准品20 00 、100 0 、500 、 250 、 125 、 62.5 、 31.2 、 0 pg /ml 为横坐标, OD 值为纵坐标,在坐标纸上作图,画出标准曲线。 3. 根据样品 OD 值在该曲线图上查出相应 NGAL 含量 ,再乘上稀释倍数即可 。 试剂盒性能 1. 灵敏度 : 最小的NGAL 检测浓度小于 15 pg/ml。 2. 特异性: 可同时检测重组或天然的人 NGAL
。因此,也有可能血清NGAL早期升高是由于插管动脉手术直接造成的。 NGAL反应的迅速 术后样本是在旁路手术开始后不同时间取样的,大家怀疑肾NGAL对损伤的反应能有多快。在已发表的临床研究中,第一个样本是在可能造成伤害的手术开始或结束1小时或2小时取样的。1小时后就可见非常明显升高,暗示甚至更短的时间也可能观察到显著升高——30分钟?15分钟?这个问题有待正式解决。但是,如果肾脏能在远远小于一小时的时间内做出NGAL反应,就要怀疑反应的引发是否可能完全是由于转录和翻译的上调造成的,可暗示预合成
大鼠Lipocalin-2 (NGAL)ELISA试剂盒 说明书
、 1000 、 500 、 250 、 125 、 62.5 、 31.2 、 0 pg/ml 为横坐标, OD 值为纵坐标,在坐标纸上作图,画出标准曲线。 3. 根据样品 OD 值在该曲线图上查出相应 NGAL 含量 ,再乘上稀释倍数即可 。 试剂盒性能 1. 灵敏度 : 最小的 NGAL 检测浓度小于 15pg/ml 。 2. 特异性: 可同时检测重组或天然的大鼠 NGAL 。 不与 大鼠
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