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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated full-length human GST.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 抗原来源:
KLH-conjugated full-length human GST.
- 目录编号:
251902
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
GST
- 抗体英文名:
GST Antibody
- 抗体名:
GST Antibody
- 规格:
0.1 mg
GST Antibody产品详细介绍:
| 产品名称: | GST Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 251902 |
| 规格: | 0.1 mg |
| 产品描述: | Enzymes of the glutathione S-transferase (GST) family are composed of many cytosolic, mitochondrial and microsomal (now designated as MAPEG) proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GSTs can constitute up to 10% of cytosolic protein in some mammalian organs. GSTs catalyse the conjugation of reduced glutathione, via a sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity detoxifies endogenous compounds such as peroxidised lipids as well as breakdown of xenobiotics. GSTs may also bind toxins and function as transport proteins, and, therefore, an early term for GSTs was “ligandin”. The mammalian GST super-family consists of cytosolic dimeric isoenzymes of 45–55 kDa size that have been assigned to at least six classes: Alpha, Mu, Pi, Theta, Zeta and Omega. GST tags are commonly used as epitope tags in recombinant protein expression and purification. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated full-length human GST. |
| 适用物种: | H |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:10,000-1:50,000; WB: 1:200-1:1,000; IHC: 1:100-1:500 |
| 别名: | Glutathione S-transferase P; GST class-pi; GSTP1-1; GST pi; GSTP1-1; GSTP1; FAEES3; GST3 |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验应用。用GST融合表达系统表达外源基因时,需要对融合表达的产物进行检测和纯化,这里面就包括了GST标签抗体的应用,如果选择一个最具性价比的GST抗体显得尤为重要。首先需要考虑抗体的来源,比如小鼠源的单克隆抗体还是兔源的多克隆抗体,一般来说,单抗的特异性、稳定性以及效价都要优于多克隆抗体。EarthOx的GST标签抗体――Anti-GST Tag Monoclonal Antibody [GST.B6](货号#E022040)是来源于小鼠的单克隆抗体,克隆号为GST.B6,免疫原为GST多肽 。
,只要互作蛋白间具有相互结合的结构域基础即可实验证明;Co-IP 实验是细胞内高表达目的蛋白,并通过 IgG 球珠-抗体-蛋白复合物体系,分离出互作的蛋白复合物,是细胞内的体内反应结合。2. GST pull-down 实验优点① 可验证蛋白质-蛋白质之间的直接互作。② GSH 谷胱甘肽偶联球珠亲和力强,洗脱纯度高。3. GST pull-down 实验缺点① 验证互作是在试管中进行的生化反应,不能够完全反应细胞内蛋白真实互作状态。② 融合表达的 GST 标签,肽链较长,可能会改变原目的蛋白的原有的折叠结构。
在免疫反应方面,基于抗原的刺激在体内形成、并与抗原作特异性结合的蛋白质之总称。其化学实质是免疫球蛋白。抗体,按照抗原抗体反应的类型可分别称为沉淀素、凝集素、溶血素、溶菌素、抗毒素、中和抗体等,但这些并不等于有相应的不同抗体存在,一般多只表明抗体反应的多样性而已。抗体在血清中含量最多(体液抗体=humoral antibody);含有某特定抗体的血清称为抗血或免疫血清。
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