- ￥3000 - 3999
- Abbiotec
- 美国
- 251861
- 2025年07月13日
- E, WB, IHC
- Rabbit
- H, R
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GST pi.
- 亚型：
Rabbit Ig
- 形态：
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件：
Store at -20°C.
- 克隆性：
多克隆
- 标记物：
见下方详细说明
- 适应物种：
H, R
- 保质期：
1-2年
- 抗原来源：
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GST pi.
- 目录编号：
251861
- 级别：
超纯
- 库存：
大量
- 供应商：
Abbiotec
- 宿主：
Rabbit
- 应用范围：
E, WB, IHC
- 浓度：
见详细说明信息
- 靶点：
GST pi
- 抗体英文名：
GST pi Antibody
- 抗体名：
GST pi Antibody
- 规格：
0.1 mg
GST pi Antibody产品详细介绍：
| 产品名称： | GST pi Antibody |
| 说明书： | 【点击查看详细说明及参考图片】 |
| 货号： | 251861 |
| 规格： | 0.1 mg |
| 产品描述： | Enzymes of the glutathione S-transferase (GST) family are composed of many cytosolic, mitochondrial and microsomal (now designated as MAPEG) proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GSTs can constitute up to 10% of cytosolic protein in some mammalian organs. GSTs catalyse the conjugation of reduced glutathione, via a sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity detoxifies endogenous compounds such as peroxidised lipids as well as breakdown of xenobiotics. GSTs may also bind toxins and function as transport proteins, and, therefore, an early term for GSTs was “ligandin”. The mammalian GST super-family consists of cytosolic dimeric isoenzymes of 45–55 kDa size that have been assigned to at least six classes: Alpha, Mu, Pi, Theta, Zeta and Omega. |
| 克隆类型： | Rabbit Polyclonal Antibody |
| 亚型： | Rabbit Ig |
| 免疫原： | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GST pi. |
| 适用物种： | H, R |
| 应用范围： | E, WB, IHC |
| 应用说明： | E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500 |
| 别名： | Glutathione S-transferase P; GST class-pi; GSTP1-1; GST pi; GSTP1-1; GSTP1; FAEES3; GST3 |
| 形态： | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件： | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献： | Qian G et al. 2013. Sage Journals. Toxicology Pathology. doi: 10.1177/0192623313477256. PMID# 23423819. |
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文献和实验，只要互作蛋白间具有相互结合的结构域基础即可实验证明；Co-IP 实验是细胞内高表达目的蛋白，并通过 IgG 球珠-抗体-蛋白复合物体系，分离出互作的蛋白复合物，是细胞内的体内反应结合。2. GST pull-down 实验优点① 可验证蛋白质-蛋白质之间的直接互作。② GSH 谷胱甘肽偶联球珠亲和力强，洗脱纯度高。3. GST pull-down 实验缺点① 验证互作是在试管中进行的生化反应，不能够完全反应细胞内蛋白真实互作状态。② 融合表达的 GST 标签，肽链较长，可能会改变原目的蛋白的原有的折叠结构。
mL PBS 1X 4- Centrifuge (1pulse at 500g) 5- Resuspend in 10 mL PBS 1X (stable for one month at 4 _ C) b) Extract preparation: 1- Transform BL21 or any appropriate bacterial strain with your GST-fused cDNA. Always use freshly transformed cells
. However, the attainable 3D resolution of an optical microscope has long been hampered by a comparatively poor resolution along the optic axis. By coherent focusing through two objective lenses, 4Pi microscopy improves the axial resolution by three- to fivefold
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