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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
mouse IgG2b
- 形态:
Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆
- 标记物:
见下方详细说明
- 适应物种:
H
- 保质期:
1-2年
- 目录编号:
251096
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
见详细说明信息
- 靶点:
CD235ab (HIR2)
- 抗体英文名:
CD235ab (HIR2) Antibody
- 抗体名:
CD235ab (HIR2) Antibody
- 规格:
2 ml
CD235ab (HIR2) Antibody产品详细介绍:
| 产品名称: | CD235ab (HIR2) Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 251096 |
| 规格: | 2 ml |
| 产品描述: | The CD235ab (HIR2) antibody recognizes a N-terminal homologous portion of glycophorin-A (GPA) and glycophorin-B (GPB), which are single-pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface acting as a barrier to cell fusion, therefore minimizing aggregation between red blood cells in the circulation. The CD235ab (HIR2) antibody agglutinates untreated RBC but failed to agglutinate papain-treated cells. This antibody primarly binds to GPA and weakly to GPB. CD235ab is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235ab positive whereas CD45 and CD71 negative. |
| 克隆类型: | Mouse Monoclonal Antibody, PE-conjugated Antibody |
| 亚型: | mouse IgG2b |
| 免疫原: | |
| 适用物种: | H |
| 应用范围: | FC |
| 应用说明: | FC with whole blood: 100 μl blood + 20 μl antibody; FC with cells: 10E6 cells + 20 μl antibody. FC: 1:200-1:1,000 |
| 别名: | CD235ab; CD235a; Glycophorin-A; PAS-2; Sialoglycoprotein alpha; MN sialoglycoprotein; GYPA; GPA; CD235B; Glycophorin-B; PAS-3; Sialoglycoprotein delta; SS-active sialoglycoprotein; GYPB; GPB |
| 形态: | Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: | Shen DC., et al. 1993. J. of Monoclonal Antibody. 9(2): 59; David M., et al. 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582. |
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107 的 PBMC 细胞用 100μl 的 Buffer 稀释(Buffer CI:含 0.5% BSA 的 PBS 缓冲液,1 mM EDTA) · 加入多种生物素化抗体,室温下孵育 5 分钟。生物素化的抗体包含以下几种,每种抗体 0.5 μg:CD235a (erythrocytes), CD14 (monocytes), CD3 (T cells), CD15 (granulocytes), CD19 (B cells), CD36 (thrombocytes), CD123
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