The CD235ab (HIR2) antibody recognizes a N-terminal homologous portion of glycophorin-A (GPA) and glycophorin-B (GPB), which are single-pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities while GPB accounts for S, s and U specificities. GPA and GPB provide the cells with a large mucin-like surface acting as a barrier to cell fusion, therefore minimizing aggregation between red blood cells in the circulation. The CD235ab (HIR2) antibody agglutinates untreated RBC but failed to agglutinate papain-treated cells. This antibody primarly binds to GPA and weakly to GPB. CD235ab is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells. Mature erythrocytes are CD235ab positive whereas CD45 and CD71 negative.
克隆类型:
Mouse Monoclonal Antibody, PE-conjugated Antibody
亚型:
mouse IgG2b
免疫原:
适用物种:
H
应用范围:
FC
应用说明:
FC with whole blood: 100 μl blood + 20 μl antibody; FC with cells: 10E6 cells + 20 μl antibody. FC: 1:200-1:1,000
Each vial contains 2 ml IgG (100 tests) in PBS pH7.4, 0.1% BSA with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
保存条件:
Store at 4°C. Product is guaranteed 6 months from the date of shipment.
参考文献:
Shen DC., et al. 1993. J. of Monoclonal Antibody. 9(2): 59; David M., et al. 2002. Leucocyte Typing VII: White Cell Differentiation Antigens. P577-582.