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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ERK1/2.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 5% BSA with 0.09% sodium azide. Antibody was purified by immunogen affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆
- 标记物:
见下方详细说明
- 适应物种:
H, M, R
- 保质期:
1-2年
- 抗原来源:
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ERK1/2.
- 目录编号:
251870
- 级别:
超纯
- 库存:
大量
- 供应商:
Abbiotec
- 宿主:
Rabbit
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
ERK1/2
- 抗体英文名:
ERK1/2 Antibody
- 抗体名:
ERK1/2 Antibody
- 规格:
0.1 mg
ERK1/2 Antibody产品详细介绍:
| 产品名称: | ERK1/2 Antibody |
| 说明书: | 【点击查看详细说明及参考图片】 |
| 货号: | 251870 |
| 规格: | 0.1 mg |
| 产品描述: | Mitogen-activated protein kinase 3 (ERK1/2) is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 is activated by tyrosine phosphorylation in response to insulin and NGF. |
| 克隆类型: | Rabbit Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ERK1/2. |
| 适用物种: | H, M, R |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500 |
| 别名: | ERK-1; Mitogen-activated protein kinase 3; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAPK1; p44-ERK1; ERT2; p44-MAPK; Microtubule-associated protein 2 kinase; MAPK3; ERK1; PRKM3; and for ERK-2; Mitogen-activated protein kinase 1; Extracellular signal-regulated kinase 2; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK2; p42-MAPK; ERT1; MAPK1; ERK2; PRKM1; PRKM2 |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 5% BSA with 0.09% sodium azide. Antibody was purified by immunogen affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
(3)同上。两个磷酸化位点都要做,但是可能在不同的病理条件下,侧重点有所不同,这主要体现在你的论文的讨论中。因此建议实验前多做论文研究,查阅一下别人的发表文章,看看与你的病理模型更相关的是哪个位点。 真爱满行囊 非常感谢你 的回复,现在我遇到的问题是我做出来蛋白总量还有216位点是有变化的,9位因为买的是santa curz的抗体,现在死活做不出来,很头痛,我要说明的问题是他的活性降低,我也看到216位点磷酸化水平降低了,可是现在没有第九位的结果(就是没有办法得到第九
并吸空预洗槽。 3)放置酶标板:放置好酶标板,酶标板行的孔数定于清洗头孔数,二者一致。 4)行选择、洗板:在控制面板上设置好要清洗的行数,按下Start键,开始洗板。 5)复位:按下控制面板上的Reset键,将在任一时间停止当前的清洗程序,系统复位到程序前状态。 6)清洗:仪器用完后,将洗液瓶、废液瓶内液体倒出,用蒸馏水将管道彻底清洗。 (2)Wellwash 4 Mk2洗板机程序卡操作 转换卡上有4个旋式和9个双向式开关,各开关的设置控制洗板机功能可完成不同的洗板程序
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