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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CREB-1.
- 亚型:
Rabbit Ig
- 形态:
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography.
- 保存条件:
Store at -20°C.
- 克隆性:
多克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
H, M, R
- 宿主:
Mouse
- 应用范围:
E, WB, IHC
- 浓度:
见详细说明信息
- 靶点:
CREB-1
- 抗体英文名:
CREB-1 Antibody
- 抗体名:
CREB-1 Antibody
- 规格:
0.1 mg
CREB-1 Antibody产品详细介绍:
| 产品名称: | CREB-1 Antibody |
| 说明书: | http://www.abbiotec.com/antibodies/creb-1-antibody-0【参考图片】 |
| 货号: | 250613 |
| 规格: | 0.1 mg |
| 产品描述: | As a dimer, CREB-1 (cAMP-responsive element-binding protein 1) binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters, to stimulate transcription. CREB-1 is activated by phosphorylayion on Ser-133 and Ser-142. Transcriptional activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB-1 has been implicated in synchronization of circadian rhythmicity. It interacts with various proteins including TORC/CRTC, CREBBP, ARRB1. |
| 克隆类型: | Mouse Polyclonal Antibody |
| 亚型: | Rabbit Ig |
| 免疫原: | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CREB-1. |
| 适用物种: | H, M, R |
| 应用范围: | E, WB, IHC |
| 应用说明: | E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500 |
| 别名: | Cyclic AMP-responsive element-binding protein 1; cAMP response element-binding protein; CREB; CREB1; CREB-1 |
| 形态: | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-G affinity chromatography. |
| 保存条件: | Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment. |
| 参考文献: |
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文献和实验antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
Column Buffers: CREB Steven Finkbeiner,Departments of Neurology and Physiology,UCSF http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/CREB_column_purification.shtml Bases Buffer is Buffer C (as per Schroter)(as per SRF)pg.27 20
of buffers prior to use. Storage Buffer for Column For 500ml 10mM Tris ph 7.5 5ml of 1M 0.3M NaCl150ml 1M or 60ml 2.5M 1mM EDTA 1ml of 0.5M 0.02% NaN3 1ml of 10% CREB Column - Purification Trial #1 Sample #5-C4-1-1 (pg.25)lysed in 4.5ml (3 vol
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