相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷藏
- 保质期:
长期
- 英文名:
Easy Cloning T-vector(pEC-T)
- 库存:
351
- 供应商:
北京百奥莱博科技有限公司
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博专业生产供应Easy Cloning T载体(pEC-T)厂家现货,我公司销*(代"售")全品类的分子生物学试剂,价格优惠,欢迎广大科研工作者垂询订购。
名称:Easy Cloning T载体(pEC-T)厂家现货
英文名:Easy Cloning T-vector(pEC-T)
产地:国产|进口
规格:20次
品牌:百奥莱博
本品是一种PCR产物高效TA克隆的专用T载体。 它由pBluescript II KS载体改造而成:保留了pBluescript II KS载 体全部的酶切位点;PCR产物插入位点处于多克隆酶切位点的中间,其两侧都有众多的酶切位点可供选择。由于本载体以pBluescript II KS 载体为基础构建而成,所以它具有同pBluescript II KS载体相同的功能:PCR产物插入后可以利用a-互补性进行蓝白斑筛选,挑选阳性克隆。可以用M13通用引物和T7,T3启动子引物对PCR产物进行测序。含有T7及T3 RNA聚合酶的启动子,可以对插入片段进行体外转录。
产品组成:
| 组份 | 规格 |
| pEC-T vector(25 ng/μl) | 100μl |
| T4 DNA Ligase | 30ul |
| 2×Quick Ligation Buffer | 500ul |
| 10×Ligation Buffer | 500ul |
| Control Insert(50 ng/μl) | 10ul |
| 附赠 | |
| 2×Taq MasterMix (快速上样型) | 1ml×2 |
| 2×Fast Taq MasterMix (快速上样型) | 1ml |
| DNA Ladder 2000 Plus | 100ul |
产品特点:
1、15分钟快速连接:
· 随酶提供独特配方的2×快速及10×普通T4 DNA Ligation Buffer。
· 使用2×快速Buffer在室温(25℃)下,仅需15分钟即可完成连接反应。
2、自带超快PCR鉴定2×Fast Taq MasterMix及DNA Marker:
2×Fast Taq MasterMix (Quick Load型)已含有PCR反应所需的快速型PCR聚合 酶、dNTP混合物、MgCl2、上样染料以及反应缓冲液预先,使用时只需再加入 模板和引物并稀释到1倍浓度即可进行PCR反应。其扩增速度为普通Taq酶的数 倍,因此可大幅度缩短PCR鉴定过程所需要的时间, 试剂盒自带预混1×Loading Buffer的DNA Ladder 2000 Plus,能满足绝大部分PCR产物电泳的需要。
质量控制:
· Control Insert克隆后的白色菌落中,有90%以上 含有Insert DNA片段。
· Control Insert经克隆后,经测序确认“T”突出的存在。
Easy Cloning T载体(pEC-T)厂家现货正在火爆热销,除此之外,为您推荐下别热销产品:
·重组人羧肽酶M(CPM)
编号:JN1767
英文名称:Recombinant Human Carboxypeptidase M
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Leu18-His422在C端含有His标签。
CPM质量控制:>95%(还原性SDS-PAGE)
CPM制剂:液体
CPM保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于CPM:
Carboxypeptidase M (CPM) specifically removes C-terminal basic residues (Arg or Lys) from peptides and proteins. Carboxypeptidase exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. CPM is believed to play important roles in the control of peptide hormone and growth factor activity at the cell surface, and in the membrane-localized degradation of extracellular proteins. It is widely distributed in a variety of tissues and cells. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
Easy Cloning T载体(pEC-T)厂家现货关键词:Easy Cloning T载体(pEC-T),Easy Cloning T-vector(pEC-T),JN0103
BTN120405 碱性磷酸酶,大肠杆菌 Alkaline Phosphatase,E.coli
四乙酰核糖 Allura Red AC 13035-61-5
BL0823 HRP标记兔抗狗IgG抗体
BTN80927 大提柱式细菌DNAOUT Maxi Column Bacterial DNAOUT
ARB11838 人凝血因子Ⅶ(FⅦ)elisa检测 Human coagulation factor Ⅶ,fⅦ ELISA KIT
ARB11890 人磷酯酰肌醇特异性磷酯酶C(PIPLC)Elisa定量检测 Human phosphoinositide-specific phospholipase c,piplc ELISA KIT
BL1189 无菌去离子水(For Cell Culture )
9001-32-5 Fibrinogen 纤维蛋白原 (牛源)
ARB13377 牛流行性出血热(EHF)ELISA检测服务
83-88-5 Vitamin B2 Riboflavin 核黄素
环磷酸腺苷*盐 EEDQ 37839-81-9
ARB11101 人金属硫蛋白(MT)代做ELISA实验 Human metallothionein,mt ELISA KIT
ARB13966 猪胰岛素样生长因子2(IGF-2)酶联免疫定量检测 Porcine insulin-like growth factors 2,igf-2 ELISA KIT
Easy Cloning T载体(pEC-T)厂家现货关键词:Easy Cloning T载体(pEC-T),Easy Cloning T-vector(pEC-T),JN0103
·重组大鼠M-CSF1
编号:JN0206
英文名称:Recombinant Rat Macrophage colony-stimulating factor 1
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物表达系统制备,目的基因编码的Gln33-Arg254表达纯化而来。
M-CSF/CSF1质量控制:>95%(还原性SDS-PAGE)。
M-CSF/CSF1制剂:冻干品
M-CSF/CSF1保存:
冻干蛋白可在-20℃长期保存,室温也可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天。
复溶后建议分装冻存在-20℃,可保存3个月,应避免反复冻融。
M-CSF/CSF1复溶:
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存,避免反复冻融。
关于M-CSF/CSF1:
Rat Macrophage colony-stimulating factor 1(MCSF,CSF1) is a single-pass type I membrane cytokine. It is a hematopoietic growth factor that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. MCSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It is involved in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development which for normal male and female fertility. It promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. MCSF also plays a role in lipoprotein clearance.
我公司生产供应销*(代"售")的克隆与表达产品正全系列现货促销,满分期待您的垂询选购Easy Cloning T载体(pEC-T)厂家现货。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Also see DNA Cloning § Making TA Vector (Crawford Lab) T-vectors are linear-blunt-ended plasmids with a few dT's added on by Taq polymerase. § Making TA Vector (Mounir Izallalen) § Direct PCR
here that the unused free nucleotides will compete with the DNA ends for the phosphatase, but empirically it seems not to matter. If you're worried about this you could EtOH ppt. and resuspend before phosphatasing. Cloning Taq polymerase PCR products by T
products by T/A cloning: Taq polymerase has an efficient terminal transferase activity that adds a single nucleotide to the 3' end of blunt ended duplex DNA. This terminal transferase activity greatly prefers adding A over adding C, G, or T, so most PCR
技术资料暂无技术资料 索取技术资料









