Recombinant Human CD40L 

Recombinant Human CD40L  (CI56)

产品说明(Description)

Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met113-Leu261 is expressed. 

Accession #: P29965

Known as: CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.

 

质量控制(Quality Control) 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity-BLI:

 

Loaded Anti-Human CD40L mAb-Fc(Cat#NC010) on Protein A Biosensor, can bind Human CD40L(Cat#CI56) with an affinity constant of 0.02 nM as determined in BLI assay.		Loaded Human CD40-Fc(Cat#CX12) on Protein A Biosensor, can bind Human CD40L(Cat#CI56) with an affinity constant of 3.29 nM as determined in BLI assay.
Loaded Human CD40-mFc(Cat#CW39) on AMC Biosensor, can bind Human CD40L(Cat#CI56) with an affinity constant of 10.2 nM as determined in BLI assay.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Measurement of autoantibodies against osteoprotegerin in adult human serum: development of a novel ELISA assay. Piec I. et al. American Society of Bone and Mineral Research. 2014.

 

 

FOR RESEARCH USE ONLY