Recombinant Human CD3 ε/CD3E

Recombinant Human CD3 ε/CD3E  (C578)

产品说明(Description)

Recombinant Human T-Cell Surface Glycoprotein CD3 epsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a 6His tag at the C-terminus. 

Accession #: P07766

Known as: T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.

 

质量控制(Quality Control) 

Purity:        Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:		Bioactivity-BLI:
Immobilized  Human CD3E-His(Cat#C578) at 10 μg/ml (100 μl/well) can bind Human Anti-CD3(Cat#NC006).  The ED50 of Human Anti-CD3(Cat#NC006) is 3-15 ng/ml.		Loaded Anti-Human/Monkey CD3E mAb-Fc(Cat#NC006) on Protein A Biosensor, can bind  Human CD3E-His(Cat#C578) with an affinity constant of 1.01 nM as determined in BLI assay.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY