Recombinant Human/Mouse/Rat Ac

Recombinant Human/Mouse/Rat Activin A  (C687)

产品说明(Description)

Recombinant Human/Mouse/Rat Activin A is produced by our Mammalian expression system and the target gene encoding Gly311-Ser426 is expressed. 

Accession #: P08476

Known as: Inhibin beta A chain;INHBA;Activin A

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:

Measured by its ability to induce SMAD signaling in 293-Activin A Res cells(Cat#XCC11). The ED50 for this effect is 1.3 ng/ml.	000000	Measured by its ability to induce SMAD signaling in 293-Activin A Res cells(Cat#XCC11). The ED50 for this effect is 1.3 ng/ml.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects.  Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release.  Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Inhibition of MTA2 and MTA3 induces mesendoderm specification of human embryonic stem cells[J]. Yu You. et al. Biochemical and Biophysical Research Communications. 2021.

Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis[J]. Gen Matsumae. et al. Bioengineering & Translational Medicine. 2021.

 

FOR RESEARCH USE ONLY