Activin A - Human/Mouse/Rat Ac

重组人/小鼠/大鼠Activin A蛋白 (PRP1042)

本品为高纯度、无标签重组蛋白，适用于多种细胞培养与发育生物学研究应用。

产品概述

重组人/小鼠/大鼠Activin A蛋白是一种由116个氨基酸组成的蛋白片段，分子量约为13.0 kDa。其氨基酸序列来源于人源Activin A（NP_002183.1，Gly311-Ser426），在大肠杆菌表达系统中重组表达而成，保持了天然蛋白的生物学特性。

关键特性

• 高纯度：经SDS-PAGE检测，纯度超过97%，确保实验的一致性和可重复性。
• 无动物源内毒素：采用LAL法检测，每1μg蛋白质中内毒素含量小于1.0 EU。
• 无标签蛋白：最大限度减少对蛋白天然构象和功能的影响。
• 广泛的种属反应性：适用于人、小鼠及大鼠来源的细胞实验系统。

产品形式与复溶建议

本品为冻干粉形式，冻干缓冲液为无菌的50mM NaCl、20mM Tris（pH 8.0）溶液。为保持蛋白最佳活性，建议使用配套复溶缓冲液将浓度复溶至不低于100 µg/mL，后续可根据具体实验体系进一步稀释使用。

保存与运输条件

• 保存条件：冻干粉应在<-20℃干燥环境下保存。复溶后可在4℃下保存2-7天，长期保存需置于<-20℃，建议添加0.1% HSA或BSA作为载体蛋白，并分装以避免反复冻融。
• 运输条件：常温运输。

生物学背景

Activin A属于TGF-β超家族，是一种通过二硫键连接的二聚体蛋白。它最初从性腺液中分离纯化，可刺激垂体释放卵泡刺激素（FSH），并在多种生理过程中发挥重要作用，包括激素分泌调节、生殖细胞与红细胞发育、性腺功能成熟等。

相关基因ID：3624；UniProt编号：P08476

产品别名

rHuActivin A; Inhibin beta A chain; INHBA; Activin A ACV-A

规格与定价

注意事项

使用前请先离心。本品仅供科研使用，不适用于人类或临床诊断。使用者应确保其应用不违反相关专利或许可规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human/Mouse/Rat Activin A Protein (PRP1042)

This product is a high‑purity, tag‑free recombinant protein suitable for a variety of cell culture and developmental biology applications.

Product Overview

Recombinant human/mouse/rat Activin A protein is a 116‑amino‑acid fragment (~13.0 kDa). The amino‑acid sequence derives from human Activin A (NP_002183.1, Gly311‑Ser426) and is expressed in an E. coli system, retaining the biological properties of the native protein.

Key Features

• High purity: SDS‑PAGE analysis shows purity >97 %, ensuring experimental consistency and reproducibility.
• Endotoxin‑free: LAL assay demonstrates <1.0 EU endotoxin per µg protein.
• Tag‑free protein: Minimizes interference with native conformation and activity.
• Broad species reactivity: Compatible with cell systems derived from human, mouse, and rat.

Product Form and Reconstitution Recommendations

The product is supplied as a lyophilized powder with a sterile reconstitution buffer containing 50 mM NaCl and 20 mM Tris (pH 8.0). To preserve optimal activity, reconstitute to a minimum concentration of 100 µg/mL using the supplied buffer; subsequent dilutions can be made according to the specific assay requirements.

Storage and Shipping Conditions

• Storage: Lyophilized powder should be stored desiccated at ≤ ‑20 °C. After reconstitution, store at 4 °C for 2–7 days; for long‑term storage keep at ≤ ‑20 °C, preferably with 0.1 % HSA or BSA as a carrier protein and aliquoted to avoid repeated freeze‑thaw cycles.
• Shipping: Ambient temperature.

Biological Background

Activin A belongs to the TGF‑β superfamily and forms a disulfide‑linked dimer. Originally purified from gonadal fluid, it stimulates pituitary release of follicle‑stimulating hormone (FSH) and participates in numerous physiological processes, including hormone secretion regulation, germ cell and erythroid development, and gonadal maturation.

Gene ID: 3624; UniProt ID: P08476

Product Synonyms

rHuActivin A; Inhibin beta A chain; INHBA; Activin A ACV‑A

Specifications and Pricing

Precautions

Centrifuge before use. This product is for research use only and is not intended for human or clinical diagnostic applications. Users must ensure that their use does not infringe any patents or licensing agreements.

Note: Information is subject to change due to product optimization or other factors; please contact Yakein Biotechnology for the latest details.