Recombinant Human CGR11/CGREF1

Recombinant Human CGR11/CGREF1  (CA79)

产品说明(Description)

Recombinant Human Cell Growth Regulator with EF Hand Domain Protein 1 is produced by our Mammalian expression system and the target gene encoding Ala20-Ile301 is expressed with a 6His tag at the C-terminus. 

Accession #: A0A4W8VYG7

Known as: Cell Growth Regulator with EF Hand Domain Protein 1; Cell Growth Regulatory Gene 11 Protein; Hydrophobestin; CGREF1; CGR11

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-Hcl,150mM NaCl,1mM GaCl₂, pH7.5.

 

质量控制(Quality Control)

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Cell Growth Regulator with EF Hand Domain Protein 1 (CGREF1) is a secreted calcium ion binding protein. CGREF1 contains two EF-hand domains and both EF-hands are required for function. Human CGREF1 is synthesized as a 301 amino acid precursor that contains a 19 amino acid signal sequence, and a 282 amino acid mature chain. CGREF1 is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. CGREF1 mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY