Recombinant Human Angiogenin/A

Recombinant Human Angiogenin/ANG/RNASE5  (C203)

 

产品说明(Description)

Recombinant Human Angiogenin is produced by our E.coli expression system and the target gene encoding Gln25-Pro147 is expressed. 

Accession #: P03950

Known as: Angiogenin; Ribonuclease 5; RNase 5; ANG; RNASE5

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Angiogenin belongs to the pancreatic ribonuclease family. Angiogenin is primarily expressed in the liver. It may act as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Angiogenin is a potent stimulator of new blood vessel formation. And Angiogenin is endocytosed and translocated to the nucleus by binding to actin on the surface of endothelial cells. Angiogenic activity is regulated by interaction with RNH1 in vivo. In addition, Angiogenin is associated with susceptibility to amyotrophic lateral sclerosis, which is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Angiogenin mutations in Hungarian patients with amyotrophic lateral sclerosis: Clinical, genetic, computational, and functional analyses[J]. Kornélia Tripolszki. et al. Brain and Behavior. 2019.

Inflammatory Chemokines MIP-1δ and MIP-3α Are Involved in the Migration of Multipotent Mesenchymal Stromal Cells Induced by Hepatoma Cells. BühlerLéo H. et al. Stem cells and development. 2015.

 

 

FOR RESEARCH USE ONLY