Recombinant Human EGF

Recombinant Human EGF  (C029)

产品说明(Description)

Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed. 

Accession #: P01133

Known as: Pro-Epidermal Growth Factor; EGF; Epidermal Growth Factor; Urogastrone

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.

 

质量控制(Quality Control) 

Purity:         Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:  Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

 

Bioactivity: Measured in a cell proliferation assay using BALB/c 3T3 cells.

                     The ED50 for this effect is 60-450 pg/ml.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Protein tyrosine phosphatase SHP2 suppresses host innate immunity against influenza A virus through regulating EGFR-mediated signalings[J]. Qingsen Wang. et al. Journal of Virology. 2021.

SIDT1-dependent absorption in the stomach mediates host uptake of dietary and orally administered microRNAs[J]. Qun Chen. et al. Cell Research. 2020.

Loss of ELF5–FBXW7 stabilizes IFNGR1 to promote the growth and metastasis of triple-negative breast cancer through interferon-γ signalling[J]. Snahlata Singh. et al. NATURE CELL BIOLOGY. 2020.

Design and Application of a High-Throughput, High-Content Screening System  for Natural Product Inhibitors of the Human Parasite Trichomonas vaginalis[J]. Jarrod B. King. et al. ACS Infectious Diseases. 2019.

MUC16 C terminal-induced secretion of tumor-derived IL-6 contributes to tumor-associated Treg enrichment in pancreatic cancer[J]. Kun Fan. et al. Cancer Letters. 2018.

Identification of T cell target antigens in glioblastoma stem-like cells using an integrated proteomics-based approach in patient specimens[J]. Rapp C. et al. Acta Neuropathol. 2017.

β-Spectrin regulates the Hippo signaling pathway and modulates the basal actin network[J]. Wong K K L. et al. Journal of Biological Chemistry. 2015.

Blockage of a miR-21/EGFR regulatory feedback loop augments anti-EGFR therapy in glioblastomas[J]. Zhang K. et al. Cancer letters. 2013.

Defective epithelial barrier function in asthma[J]. Chang Xiao. et al. J Allergy Clin Immunol. 2011.

SARS-CoV-2 infects and replicates in cells of the human endocrine and exocrine pancreas[J]. Janis A Müller. et al. Nature Metabolism. 2021.

 

FOR RESEARCH USE ONLY