EGF/Urogastrone/URG,Human,Recombinant

EGF/Urogastrone/URG,Human,Reco

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  • ¥400 - 1880
  • Procell
  • PCK001
  • 武汉
  • 2025年07月15日
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    • 详细信息
    • 技术资料
    • 供应商

      武汉益普生物科技有限公司

    • 英文名

      EGF/Urogastrone/URG,Human,Recombinant

    • 规格

      10μg/50ug/500ug/1mg

    规格:10μg产品价格:¥400.0
    规格:50ug产品价格:¥780.0
    规格:500ug产品价格:¥1280.0
    规格:1mg产品价格:¥1880.0

    产品信息

    别名Urogastrone, URG
    物种Human
    表达宿主E.coli
    序列信息MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus
    检索号P01133.2
    分子量7.16 kDa
    表观分子量9-11 kDa
    标签His-tag at the C-terminus
    生物活性Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 10^6 IU/mg.

     

    产品特性

    纯度>95% as determined by SDS-PAGE. Ni-NTA chromatography.
    内毒素<0.1 EU per 1 μg of the protein by the LAL method.
    保存Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
    运输Ambient temperature or ice pack.
    制剂The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
    复融It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

     

    背景介绍

    Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

    EGF/Urogastrone/URG,Human,Reco

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