
双丙氨膦
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Bialaphos
MOLECULAR BIOLOGY GRADE
NaC11H21N3O6P
MW: 345.26 g/mol
Bialaphos is a tripeptide antibiotic that is used in transformation experiments of many species of plants that make use of the bar gene for selection. In corn it is more effective than glufosinate. In wheat, bialaphos was the most reliable selection criteria used to identify bar transformed plants.
Soluble in water
Store dessicated at -20oC
MOLECULAR BIOLOGY GRADE
NaC11H21N3O6P
MW: 345.26 g/mol
Bialaphos is a tripeptide antibiotic that is used in transformation experiments of many species of plants that make use of the bar gene for selection. In corn it is more effective than glufosinate. In wheat, bialaphos was the most reliable selection criteria used to identify bar transformed plants.
Soluble in water
Store dessicated at -20oC
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文献和实验相关实验
草胺膦(Sigma) 或 2 mg/L 双丙氨膦(Meiji Seika Kaisha, Japan) 。 ( 5 ) MS5(高选择压的芽增殖培养基): MS2 培养基,10 mg/L 草胺膦或 3 mg/L 双丙氨膦。 ( 6 ) MS6(芽再生培养基):MS 培养基,20 g/L 蔗糖,0.5 mg/L N6-苄基腺嘌呤,0.5 mg/L B 吲哚三丁酸(Sigma) ,15 mg/L 草胺膦或 5 mg/L 双丙氨膦,3~5 g/L phytagel,pH
剂草丁膦和双丙氨膦;epsps, 草甘膦抗性标记基因,抗草甘膦;als,绿黄隆抗性标记基因。3. 显色或发光报告基因(1)GUS酶活性检测GUS使β-葡萄糖苷酶,能催化裂解一系列的葡萄糖苷,产生一系列具有发色基团或发荧光的物质,可用分光光度计、荧光计或组织化学法对GUS活性进行定量和空间定位分析,检测方法简单灵敏。(2)荧光素酶活性检测荧光素酶催化的底物使6-羟基喹啉类似物,在镁离子、ATP及氧的作用下酶使底物脱羧,生成激活态的氧化荧光素,发射光子后,转变成常态的氧化荧光素。(3)绿色
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双丙氨膦
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