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- 文献和实验
- 技术资料
- 保存条件：
咨询
- 英文名：
代谢相关检测
- 库存：
100
- 供应商：
上海三抒生物科技有限公司
- CAS号：
咨询
- 规格：
100ml
Alanine Racemase，中文名为丙氨酸消旋酶，催化L-丙氨酸与D-丙氨酸之间相互转化。本产品为大肠杆菌表达的丙氨酸消旋酶，并经简单纯化获得的可以确保达到一定酶活力的粗酶液。
由于本产品需新鲜制备，确认订购后约6个工作日才可以发货。
本产品可以提供十公斤、百公斤乃至吨级的粗酶液，如有需求请联系三抒生物。
本产品也可以纯化并制备成酶的冻干粉，提供量可以从克级至公斤级，如有需求请联系三抒生物。
This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on amino
acids and derivatives. This enzyme participates in alanine and aspartatemetabolism and D-alanine metabolism. It employs one cofactor, pyridoxal phosphate. At least two compounds, 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit this enzyme.The D-alanine produced by alanine racemase is used for peptidoglycan biosynthesis. Peptidoglycan is found in the cell walls of all bacteria, including many which are harmful to humans. The enzyme is absent in higher eukaryotes but found everywhere in prokaryotes, making alanine racemase a great target for antimicrobial drug development.Alanine racemase can be found in some invertebrates.Bacteria can have one (alr gene) or two alanine racemase genes. Bacterial species with two genes for alanine racemase have one that is continually expressed and one that is inducible, which makes it difficult to target both genes for drug studies. However, knockout studies have shown that without the alr gene being expressed, the bacteria would need an external source of D-alanine in order to survive. Therefore, the alr gene is a feasible target for antimicrobial drugs. This product with the indicated enzyme activity was briefly purified from engineered E.coli.
产品详细信息见下表:
| About this product | |
| Name | Alanine Racemase (Crude Enzyme)；丙氨酸消旋酶(粗酶液) |
| Application | 催化L-丙氨酸与D-丙氨酸之间相互转化 |
| Appearance | Clear to translucent yellow solution |
| Enzyme activity | Undetermined |
| About this enzyme | |
| Name | Alanine Racemase；丙氨酸消旋酶 |
| CAS Number | 9024-06-0 |
| EC Number | 5.1.1.1 |
| Enzymatic Reaction | L-alanine = D-alanine |
| Synonyms | L-alanine racemase |
| Systematic name | alanine racemase |
| Cofactor(s) | pyridoxal 5'-phosphate |
| Application | drug development; pharmacology; medicine; pharmacology |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| P3807-100ml | Alanine Racemase (Crude Enzyme) | 100ml |
| P3807-1L | Alanine Racemase (Crude Enzyme) | 1L |
| — | 说明书 | 1份 |
保存条件：
-20℃或更低温度保存，至少1个月有效。
注意事项：
由于本产品需新鲜制备，在您确认订购后约6个工作日才能发货。
粗酶液的每次冻融均可能会引起部分失活，所以收到酶液后请根据需要进行分装，并置于-20℃或更低温度保存。
随着保存时间的延长，酶活力会有一定程度的下降，收到产品后应尽快使用。
本产品在生产和保存的过程中可能会有混浊或沉淀产生，融化后混匀即可，不影响正常使用。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
上海三抒生物科技有限公司是一家从事于生物化学行业 的企业，公司致力于为实验室分析领域提供一站式服务，为用户提供专业、快捷、可靠的全方位服务。
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文献和实验Preparation and Assay of Recombinant Serine Racemase
Serine racemase is a glial and neuronal enzyme that reversibly converts l -serine to d -serine, an endogenous co-agonist of N -methyl-d -aspartate receptor type glutamate receptors (NMDARs). Here we present methods to recombinantly express
Alanine Transaminase Activity Assay
] OxiRed™ [ Detail ： in DMSO solution] 实验步骤 1. Reagent preparation: 1) Alanine Transaminase Enzyme Mix: Reconstitute with 220 µl dH2O. Aliquot and store at -20oC. Use within two months.
Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids
acid: the first one employing a multi-enzymatic redox system, the second one combining an hydrolytic enzyme together with a base-catalyzed substrate racemization. In both cases, the final product, l -3-(2-naphthyl)alanine, is recovered with good yield
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