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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
+4°C 干燥
- 保质期:
12个月
- 英文名:
Pitstop® 1 - negative control
- 供应商:
上海汇谱仪器有限公司
- 规格:
5 mg
概述
- 产品名称
Pitstop® 1 - negative control - 描述
Negative control for clathrin inhibitor Pitstop® 1 (ab120685) - 生物学描述
Negative control for clathrin inhibitor Pitstop® 1 (ab120685) which is of the same chemical class and has a highly related structure to Pitstop® 1. Inhibits amphiphysin binding to the clathrin TD with an IC50 >100 μM and does not block receptor-mediated endocytosis at concentrations of up to 300 μM (unpublished reports). - 纯度
> 98% - 常规说明
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. Pitstop® is a trademark of Freie Universitat Berlin, Newcastle Innovation Ltd. and Children's Medical Research Institute
性能
- 化学名称
2-(4-Aminobenzyl)-1,3-dioxo-2,3-dihydro-1H-benzo[de]isoquinoline-6-sulfonic acid potassium salt - 分子量
420.48 - 化学结构

- 分子式
C19H13KN2O5S - 存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. - 溶解度概述
Soluble in DMSO to 100mM and in water to 25mM - 处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
- 来源
Synthetic
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文献和实验-7 cells were transfected with CDK2 siRNA or a negative control siRNA using the indicated volumes of transfection agent per well. 48 hr after transfection, the cells were harvested and analyzed by real-time RT-PCR using TaqMan® Gene Expression Assays
Transfection of siRNA. Cos-7 cells were transfected with CDK2 siRNA or a negative control siRNA using the indicated volumes of transfection agent per well. 48 hr after transfection, the cells were harvested and analyzed by real-time RT-PCR using TaqMan® Gene
Determining Relative Transduction of Adherent Cells
实验原理 MISSION® TurboGFP™ control particles enable fast determination of the appropriate transduction conditions for efficient transduction of cells with lentiviral particles. Note: The CMV promoter is known to function
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