Description |
Peripheral Blood Mononuclear Cells (PBMCs), also known as Human Mononuclear Cells from Peripheral Blood (HMNC-PB) are generated through the hematopoiesis. PBMC is a group of blood cells of immune system, having a single and round nucleus. They are critical to human body immunity. PBMC isolation is usually processed through density gradient centrifugation of the peripheral blood. 外周血单个核细胞(Peripheral Blood Mononuclear Cells, PBMCs),又称人类外周血单个核细胞(Human Mononuclear Cells from Peripheral Blood, HMNC-PB)是通过造血产生的细胞。PBMC是免疫系统的一组血细胞,细胞核单圆形。它们对人体免疫至关重要。PBMC的分离通常采用外周血密度梯度离心的方法。 PBMC Composition Human PBMC is composed of around 70-90% different lymphocytes (T cells, B cells, and NK cells), 10-20% dendritic cells, and 1-2% monocytes. As a component of innate and adaptive immune system, PBMCs are widely used in clinical research and applications such as: immunology, hematological malignancies, infectious disease, vaccine development, transplant immunology. Besides, PBMCs can also be used in high-throughput screening, proteomics, toxicology and pre-clinical research. Cryopreserved PBMCs can be used for positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. PBMC组成 人PBMC由大约70-90%不同的淋巴细胞(T细胞、B细胞和NK细胞)、10-20%的树突状细胞和1-2%的单核细胞组成。 作为先天和适应性免疫系统的组成部分,PBMCs被广泛应用于免疫学、血液系统恶性肿瘤、传染病、疫苗研发、移植免疫学等临床研究和应用。此外,PBMCs还可用于高通量筛选、蛋白质组学、毒理学和临床前研究。低温保存的PBMCs可用于ELISPOT、ELISA、细胞因子珠阵列、四聚体/五聚体和流式细胞仪检测T细胞的阳性和阴性对照。 |