The adipocyte precursor cells (preadipocytes) are isolated from subcutaneous adipose tissue of healthy non-diabetic donors between 18 and 60 years old undergoing elective surgery. The preadipocytes are isolated by centrifugal force after collagenase treatment. Preadipocytes can be cultured as growing precursor cells or differentiated into adipocytes using medium supplemented with adipogenic and lipogenic hormones. 脂肪细胞前体细胞从18 - 60岁接受择期手术的健康非糖尿病供者的皮下脂肪组织中分离。胶原酶处理后用离心力分离前脂肪细胞。前脂肪细胞可以作为生长前体细胞培养,也可以在添加成脂和成脂激素的培养基中分化成脂肪细胞。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.