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G-CSF

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  • cellapy
  • G-CSF
  • 2026年04月02日
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    Source E. coli
    Description Recombinant Human Granulocyte Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Thr31-Pro204 is expressed.
    Names Granulocyte Colony-Stimulating Factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF
    Accession # P09919-2
    Formulation Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
    Shipping The product is shipped at ambient temperature.
    Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    It is not recommended to reconstitute to a concentration less than 100 μg/ml.
    Dissolve the lyophilized protein in ddH2O.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
    Biological Activity ED50 is less than 0.1 ng/ml. Specific Activity of 6.0 x 10^7 IU/ mg, measured by the dose-dependent proliferation of murine NFS-60 indicator cells.
    Purity Greater than 95% as determined by reducing SDS-PAGE.
    Endotoxin Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
    Amino Acid Sequence
    MTPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSC PSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPA LQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP
    Background Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.

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    图标文献和实验
    相关实验
    • Hematopoietic Stem Cell Mobilization with G-CSF

      stem cells are collected after G-CSF administration. In this chapter we describe peripheral blood stem cell mobilization in autologous transplant patients and in allogeneic donors using G-CSF.

    • Hematopoietic Stem Cell Mobilization with Agents Other than G-CSF

      factor (G-CSF) are the preferred source for transplantation. G-CSF mobilization regimens, however, are associated with known morbidities and a significant number of normal donors and patient populations fail to mobilize sufficient numbers of hematopoietic

    • 人粒细胞集落刺激因子(G-CSF)ELISA试剂盒 说明书

        上海西唐生物科技有限公司   021-55229872,  65333639   www.westang.com 人粒细胞集落刺激因子 (G-CSF)ELISA 试剂盒   ( 用于血清、血浆、细胞培养上清液和其它生物体液内 )   原理 本实验采用双抗体夹心  ABC-ELISA 法。用抗人   G-CSF   单抗包被于酶标板上,标准品和样品中的   G-CSF与单抗结合,加入生物素化的抗人

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