Recombinant Human G-CSF (C002)
产品说明(Description)
Recombinant Human Granulocyte Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Thr31-Pro204 is expressed.
Accession #: P09919-2
Known as: Granulocyte Colony-Stimulating Factor; G-CSF; Pluripoietin; Filgrastim; Lenograstim; CSF3; C17orf33; GCSF
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at ≤ -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Human Granulocyte-Colony-Stimulating Factor (G-CSF) is 20 kD glycoprotein containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and it functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1, and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17.
电泳(SDS-PAGE)
参考文献(Reference)
The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis[J]. Qiong Wang. et al. Cell Reports. 2020.
Oncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation. Welte T. et al. Nature Cell Biology. 2016.
Comparative Effects of Metal-Catalyzed Oxidizing Systems on Carbonylation and Integrity of Therapeutic Proteins. Kryndushkin D. et al. Pharmaceutical research. 2015.
FOR RESEARCH USE ONLY