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上海恪敏生物科技有限公司
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文献和实验the AllPrep DNA/RNA 96 Kit. Six targets (195-1150 bp) were amplified by multiplex PCR and then analyzed on the QIAxcel system. Li: liver; Ki: kidney; Lu: lung; In: intestine; Sp: spleen; NTC: no template control (shows upper and lower markers). Total
Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
DNA markers going from 2kb down to 100 bp or less (recommend BM PCR markers). View on UV light box at 254 - 300 nm, photo 1 - 5 sec. NOTE: Small products are best seen on 3% agarose gels that have been run fast (eg: 100 volts), with BPB run to ½
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