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文献和实验SequalPrep Long PCR Kit with dNTPs
实验步骤 1. Program the thermal cycler as follows (note that the annealing temperature will vary depending on the Tm of your primers): 94°C 2 minutes 10 cycles
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
2.1. 3' RACE LaNe 1. 20 ng total RNA (see Note 1). 2. 10 mM dNTPs. 3. ThermoScript ® Reverse Transcriptase and accompanyingbuffers (Invitrogen) (see Note 2). 4. TTT3 primer: 5'-gene-specific sequence followed byTTTTTTTTTTTTTTTTV
for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended
