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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human AKT1 / PKB / PKBα Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/20.00 µg/50.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥26795.0 |
|---|---|---|---|
| 规格: | 20.00 µg | 产品价格: | ¥1790.0 |
| 规格: | 50.00 µg | 产品价格: | ¥3220.0 |
蛋白名称:Human AKT1 / PKB / PKBα Protein (His Tag)
蛋白构建:A DNA sequence encoding the human AKT1 (NP_001014431.1) (Met 1-Ala 480) was fused with a polyhistidine tag at the C-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 87 % as determined by SDS-PAGE
蛋白活性:No Kinase Activity
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met 1
蛋白分子量:The recombinant human AKT1 consists of 491 amino acids and migrates as an approximately 57 kDa band as predicted in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001014431.1
蛋白氨基酸序列:Met1-Ala480
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Wie J, et al. A growth-factor-activated lysosomal K+ channel regulates Parkinson's pathology.Nature, PubMed ID: 33505021
3, Zhao A, et al. Elucidation of anti-pneumonia pharmacodynamic material basis and potential mechanisms of Xiebai San by combining spectrum-efficacy relationship and surface plasmon resonance.Journal of ethnopharmacology, PubMed ID: 39053707
-1. In addition to the forkhead and NF-KappaB families, E2F-mediated transcription can also be activated by the hyperphosphorylation and subsequent inactivation of Rb (Retinoblastoma Protein) in response to signals from PI3K and its downstream effectors, PKB
.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
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