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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Human RANKL / OPGL / TNFSF11 / CD254 Protein (ECD, Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg/500 µg
| 规格: | 50 µg | 产品价格: | ¥2480.0 |
|---|---|---|---|
| 规格: | 500 µg | 产品价格: | ¥16180.0 |
蛋白名称:Human RANKL / OPGL / TNFSF11 / CD254 Protein (ECD, Fc Tag)
蛋白构建:A DNA sequence encoding the human TNFSF11 (AAC51762.1) (Gly64-Asp245) was fused with the Fc region of human IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 86 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF11B-His (Cat:10271-H08H) at 10 μg/ml (100 μl/well) can bind human Fc-TNFSF11 (Cat:11682-H01H) with a linear range of 3.125-200 ng/mL. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 7-35 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu
蛋白分子量:The recombinant human TNFSF11/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 443 amino acids and has a calculated molecular mass of 48.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFSF11/Fc monomer is approximately 52 kDa due to the glycosylation.
蛋白NP号:AAC51762.1
蛋白氨基酸序列:Gly64-Asp245
蛋白标签:N-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
the isolation of activated Fc receptor complexes from RAW 264.7 macrophages using live?cell affinity receptor chromatography (LARC). The Fc receptor complex is activated and captured by IgG?coated microbeads on the surface of live macrophages. After the cells
几年才相继发现和确认.这类受体分属于MHC型和非MHC型。前 者一般与相对应的抑制性受体相互交联,如KIR家族中的KIR2DS和KIR3DS,其胞质区缺乏ITIM结构,但跨膜区有带正电的赖氨酸,此为KIR2DS提供了与DAPl2结合的分子基础。DAPl2的结构与FcγR以及CD3分子中ζ链十分相似,胞质区带有ITAM,从而使KIR2DS-DAPl2复合物能够传递活化激活信号。 非MHC型受体主要包括天然细胞毒受体(NCR)、NKG2D以及一些辅助性受体。NCR包括三类
x 106/ml; 3.4 加入1 000 U/ml 的重组人IFN-g培养; 3.5 24h 后加入50ng/ml 的CD3 单克隆抗体和300 U/ml 的重组人IL-2,刺激CIK 细胞的生长和增殖; 注:此时也可同时加入100 U/ml的重组人IL-1a。 3.6 每3天半量换液或扩瓶一次,并补加重组人IL-2 300 U/ml; 3.7 在培养的第7d,收获CIK细胞,此时数量应达到1x 109个以上。 3.8 CIK细胞质控: 3.8.1 台盼蓝染色检测
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