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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Influenza B (B/Malaysia/2506/2004) HA1 (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建:A DNA sequence encoding the N-terminal segment (Met 1-Arg 362) of the influenza hemagglutinin (B/Malaysia/2506/2004) (ACO05957.1), termed as HA1, was fused with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asp 16
蛋白分子量:The secreted recombinant HA1 subunit of influenza B HA (B/Malaysia/2506/2004) comprises 358 amino acids and has a predicted molecular mass of 39 kDa. As a result of glycosylation, it migrates as an approximately 55-60 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:ACO05957.1
蛋白氨基酸序列:Met1-Arg362
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Khan, S; et al. Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray. BioRxiv
The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
Katz, J.M., Lu, X., Tumpey, T.M., Smith, C.B., Shaw, M.W., and Subbarao, K. 2000. Molecular correlates of influenza A H5N1 virus pathogenesis in mice. J. Virol. 74:10807‐10810.
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
蛋白标签(protein tag)是指利用DNA体外重组技术,与目的蛋白一起融合表达的一种多肽或者蛋白,以便于目的蛋白的表达、检测、示踪和纯化等。随着技术的不断发展,研究人员相继开发出了具有各种不同功能的蛋白标签。 一、 新型蛋白标签 蛋白标签应用极为广泛,但仍有两个问题不容忽视:(1)蛋白标签以DNA形式编码,它需要转录并翻译成蛋白后才能发挥作用,而这种作用方式本身就是一种缺陷。例如,常用的荧光蛋白标签,在显微镜下观察时,其显像不如人工合成的荧光基团好,但是这种人工合成的荧
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