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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ 至 -80℃
- 保质期:
12个月
- 英文名:
Influenza A H5N1 (A/Japanese white-eye/Hong Kong/1038/2006) Hemagglutinin Protein (HA1 Subunit) (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建:A DNA sequence encoding the N-terminal segment (Met 1-Glu 340) of the influenza A hemagglutinin (A/Japanese white-eye/Hong Kong/1038/2006 (H5N1)) (ABJ96775.1), termed as HA1, was fused with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asp 17
蛋白分子量:The secreted recombinant HA1 subunit of influenza A H5N1 HA (A/Japanese white-eye/Hong Kong/1038/2006 (H5N1)) comprises 335 amino acids and has a predicted molecular mass of 38 kDa. As a result of glycosylation, it migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:ABJ96775.1
蛋白氨基酸序列:Met1-Glu340
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验of complex and protein alone with the naked eye or a light microscope, crystals of covalently Cy5?labeled DNA can be directly used for X?ray diffraction data collection. Curr. Protoc. Nucleic Acid Chem. 46:7.15.1?7.15.8. © 2011 by John Wiley & Sons
蛋白磷酸化就在有丝分裂、细胞死亡、DNA 损伤修复、DNA 复制和重组过程中发挥着直接的作用。 组蛋白翻译后修饰多发生在组蛋白的 N-端尾部,包括甲基化、乙酰化、磷酸化、ADP-核糖基化、泛素化和小分子泛素化修饰,这些修饰有助于其他蛋白质与 DNA 的结合,从而产生协同或者拮抗作用来调控基因转录。例如,乙酰化使组蛋白尾部正电荷减少,从而削弱了与带负电荷 DNA 骨架的作用,而促进染色质呈开放状态, 甲基化激活或抑制基因功能主要依赖于修饰的位点,主要与赖氨酸残基的单甲基化、双甲基化或三甲基化有关。 组蛋白修饰最基本
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