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- Sino Biological
- 50457-M03H
- 2026年01月26日
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- 规格：
100 µg
蛋白名称：Prolactin Receptor蛋白, Prolactin Receptor protein
蛋白构建：A DNA sequence encoding the extracellular domain of mouse PRLR (NP_035299.4) (Met 1-Asp 229) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 21
蛋白分子量：The recombinant mouse PRLR/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 457 amino acids and has a calculated molecular mass of 52.5 kDa. As a result of glycosylation, the apparent molecular mass of the mouse PRLR/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_035299.4
蛋白氨基酸序列：Met1-Asp229
蛋白标签：C-human IgG1-Fc & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Clonality - X Chromosome Inactivation Assay
and can assist in interpretation of allele patterns. 4. References Allen RC, Zoghbi HY, Moseley AB, Rosenblatt HM, Belmont JW. Methylation of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome
HIS 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的 HIS 标签蛋白。该问题主要可以分为以下两个方面： 1、HIS 标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螫合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲
Ligand- and Structure-Based Pregnane X Receptor Models
The human pregnane X receptor (PXR) is a ligand dependent transcription factor that can be activated by structurally diverse agonists including steroid hormones, bile acids, herbal drugs, and prescription medications. PXR regulates
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