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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
一年
- 英文名:
Western Hybridization Service
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
1
Western 杂交是1979年由美国科学家Stark和瑞士科学家Towbin独立发明的、专门用于检测固定在固相基质上的蛋白质的免疫方法,又叫免疫印迹(immunoblotting)。该方法的是先将蛋白质从凝胶中转移到固相基质(硝酸纤维素膜或尼龙膜)上;再对其进行特异性抗体检测。Western杂交流程图如下:
病毒DNA/RNA共提取试剂盒
R6872-01 HP Viral DNA/RNA Kit(4x96)
R6872-02 HP Viral DNA/RNA Kit(12x96)
R6873-01 HP Viral DNA/RNA Kit(50)
R6873-02 HP Viral DNA/RNA Kit(200)
微量病毒DNA/RNA共提取试剂盒
R6974-01 MicroElute Viral DNA/RNA Kit(50)
R6974-02 MicroElute Viral DNA/RNA Kit(200)
M6245-01 Mag-Bind Viral DNA/RNA Kit(50)
Western杂交技术服务-Western Hybridization ServiceM6245-02 Mag-Bind Viral DNA/RNA Kit(200)
磁珠DNA/RNA共提取试剂盒
M6246-01 Mag-Bind Viral DNA/RNA Kit(1x96)_
M6246-02 Mag-Bind Viral DNA/RNA Kit(4x96)
磁珠MRNA提取试剂盒:不带总RNA提取试剂
R6520-01 Mag-Bind mRNA Enrichment Kit(10)
R6520-02 Mag-Bind mRNA Enrichment Kit(30)
磁珠MRNA提取试剂盒:
R6570-01 Mag-Bind mRNA Kit(10)
R6570-02 Mag-Bind mRNA Kit(30)
Oligo纤维素Mrna抽提试剂盒
R6511-01 mRNA Enrichment Kit (10 preps)
R6511-02 mRNA Enrichment Kit (30 preps)
粪便RNA提取试剂盒
R6828-00 Stool RNA Kit(5)
R6828-01 Stool RNA Kit(50)
R6828-02 Stool RNA Kit(200)
mRNA 提取试剂盒
R6842-00 mRNA Kit(5)
R6842-01 mRNA Kit(50)
R6842-02 mRNA Kit(200)
植物DNA\RNA 共提取试剂盒
R6733-00 Plant DNA\RNA Kit(5)
R6733-01 Plant DNA\RNA Kit(50)
R6733-02 Plant DNA\RNA Kit(200)
DNARNA蛋白质共提取试剂盒
R6734-00 DNARNAProtein Kit(5)
R6734-01 DNARNAProtein Kit(50)
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文献和实验for the hybridization chamber. One can also cut the sheet into strips, etc. 5. Wash the sheet in TS for 10 minutes, twice in TSN for 20 minutes each, and then again in TS for 10 minutes. When higher stringency is required, wash blot 4 times 15-20 minutes in blocking
METHOD for Western Blots:1.While your SDS-PAGE gel is running,make your transfer buffer and chill to 4℃.Check that you have a frozen buffer dam is ready (stored in freezer next to Shikha,top shelf to the right side--looks like rectangular white
with transfer module--prefill to about 2/3 with of cold transfer buffer (For the biorad system, you use the same tank that you ran your gels in, but with a red and black transfer module that lives in the drawer (gel room, third drawer under the hybridization
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