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- 保存条件:
低温运输,-20℃保存
- 保质期:
一年
- 英文名:
Alkaline Phosphatase,E.coli
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
25 U/盒
产品及特点:
Bacterial Alkaline Phosphatase(BAP)大肠杆菌碱性磷酸酶是来源于大肠杆菌的碱性磷酸酶(BAP),本酶催化所有磷酸单酯的水解,不能催化磷酸二酯及磷酸三酯的水解,但能催化ATP等焦磷酸键的水解。它具有下列特点:
1. 在用32P标记5′-末端之前,去除RNA或DNA的5′-末端的磷酸基。
2. 去除载体DN**段的 5′-末端磷酸基。防止线状DNA自身环化(Selfligation)。活性单位定义在 25℃、pH8.0 的条件下,1 分钟内水解对**苯磷酸盐( p-nitrophenylphosphate )生成 1 μ mol 的对**苯酚(p-nitrophenol)所需的酶量定义为1个活性单位(U)。
纯度
1. 1 U的本酶和1 μg的λDNA-Hind III片段在37℃下反应16小时,DNA的电泳谱带不发生变化。
2. 1 U的本酶和1 μg的16S,23S rRNA在37℃下反应16小时,RNA的电泳谱带不发生变化。
碱性磷酸酶,大肠杆菌-Alkaline Phosphatase,E.coli备注
本酶是一种稳定性很高、每一个酶分子中含有2个Zn2+的金属蛋白质。在螯合剂存在的条件下,100℃加热可暂时性失活,但由于恢复至室温时酶活性可以恢复,因此必须进行苯酚处理,才能使其完全失活。本酶最适pH在8.0附近,Tris等醇类化合物可使其活性提高。D6492-00 Cycle Pure Kit(5)-S Spin Column
D6492-01 Cycle Pure Kit(100)-S Spin Column
D6492-02 Cycle Pure Kit(200)-S Spin Column
D6493-00 Cycle Pure Kit(5)-Q Spin Column
D6493-01 Cycle Pure Kit(100)-Q Spin Column
D6493-02 Cycle Pure Kit(200)-Q Spin Column
96孔板PCR产物纯化试剂盒:40分钟内纯化96个PCR产物
D1043-01 E-Z 96 Cycle Pure Kit(1x96)
D1043-02 E-Z 96 Cycle Pure Kit(5x96)
超薄柱型PCR产物纯化试剂盒:用低洗脱体积纯化或浓缩PCR产物、酶切产物
D6293-01 MicroElute Cycle Pure Kit(50)
D6293-02 MicroElute Cycle Pure Kit(200)
磁珠纯化PCR产物
M1322-01 Mag-Bind Cycle Pure Kit(4x96)
M1322-02 Mag-Bind Cycle Pure Kit(24x96)
DNA纯化试剂盒:数分钟内纯化DNA酶促产物
微量DNA纯化试剂盒:从各种粗制的DNA模板中进一步纯化或浓缩基因组DNA
D6296-01 MicroElute DNA Clean Up Kit(50)
D6296-02 MicroElute DNA Clean Up Kit(200)
磁珠法自动测序染料去除试剂盒:高通量地去除自动测序前的游离荧光染料探针
M1320-01 Mag-Bind Dye Terminator Removal Kit(4x96)
M1320-02 Mag-Bind Dye Terminator Removal Kit(24x96)
DNA探针纯化试剂盒:从标记反应中快速回收DNA探针
D6538-00 DNA Probe Purification Kit(5)
D6538-01 DNA Probe Purification Kit(50)
D6538-02 DNA Probe Purification Kit(200)
离心型染料去除试剂盒:用葡聚糖凝胶去除自动测序前的游离染料
S5912-00 Ultra-Sep Dye Terminator Removal Kit(5)
S5912-01 Ultra-Sep Dye Terminator Removal Kit(50)
S5912-02 Ultra-Sep Dye Terminator Removal Kit(200)
磁珠聚核苷酸纯化试剂盒
M2513-01 Mag-Bind Oligonucleotide Purification Kit(4 x 96)
M2513-02 Mag-Bind Oligonucleotide Purification Kit(20 x 96)
M2514-01 Mag-Bind Oligonucleotide Purification Kit(50)
M2514-02 Mag-Bind Oligonucleotide Purification Kit(200)
凝胶回收试剂盒
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文献和实验Purification of acidic phosphatase from mustard seedlings
at which such phosphatases have optimal activity, we distinguish between acidic phosphatases (also called acid phosphatases) and alkaline phosphatases. The latter enzymes require divalent metal ions as cofactors and are common in animal tissues and bacteria. Acidic
Purification of acidic phosphatase from mustard seedlings
at which such phosphatases have optimal activity, we distinguish between acidic phosphatases (also called acid phosphatases) and alkaline phosphatases. The latter enzymes require divalent metal ions as cofactors and are common in animal tissues and bacteria. Acidic
Expression of a cDNA in E.coli in order to prepare an antiserum
an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase ( LacZ
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