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- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
低温运输,4℃保存
- 保质期:
一年
- 英文名:
Phosphatase Alkaline(E.coli)
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
5 mg/盒
来源:E.coli
活力:≥20 Units per mg protein
用途:生化研究。切除DNA, RNA, rNTPs及dNTPs的5′末端磷酸基团;防止克隆载体的自环化连接;蛋白质丝氨酸、苏氨酸、酪氨酸残基的去磷酸化。
碱性磷酸酶(大肠杆菌)-Phosphatase Alkaline(E.coli)低温运输,4℃保存,有效期一年。1.2ml 8-Strip tubes,Presterile,960 tubes
1.2ml 12-Strip tubes,Non-sterile,960 tubes
1.2ml 12-Strip Tubes,Presterile,960 tubes
AeraSeal Film(25)
AeraSeal Film(50)
Sealing film(25)
Sealing film(100)
Multi-Channel Diposable reservoir(10pk)
12-channel reservoir(5/pk.4pk/case)
E-Z 96 Lysate Clearance Plate(10)
E-Z 97 Lysate Clearance Plate(100)
Homogenizer Spin Column(200)
EZ 96 Homogenization plate(4)
EZ 96 Homogenization plate(1)
Rnase Free Dnase Set(50)
RNase A(400ul)
RNase A(5ml)
Proteinase K,30mg
Proteinase K,100mg
Proteinase K,1g
OB Protease,30mg
OB Protease,100mg
Lysozyme A(2500U)
Lysozyme A(10000U)
LyticaseA(2500U)
LyticaseA(10000U)
0.1mm-0.2mm Glass Beads(50g)
0.1mm-0.2mm Glass Beads(500g)
0.4mm-0.6mm Glass Beads(50g)
0.4mm-0.6mm Glass Beads(500g)
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文献和实验Expression of a cDNA in E.coli in order to prepare an antiserum
an antiserum. As an alternative, the cDNA can be expressed in E.coli as a translational fusion and the expressed protein used to prepare the antiserum. This is normally done as fusion constructs with e.g. -galactosidase ( LacZ
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
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