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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
一年
- 英文名:
BSA Blocking Solution
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
250 mL/盒
产品及特点
本产品可用于所有封闭应用,包含A 型Blocker BSA in PBS(10×)和 B 型Blocker BSA in TBS(10×)。本产品具有下列特点:
1. 10% 高质量牛血清白蛋白溶液。
2. 浓缩工艺节省存储空间。
3. 液体浓缩液稀释后即可使用,无须等待粉末的溶解。
牛血清白蛋白封堵液-BSA Blocking Solution低温运输,-20℃保存,有效期一年。TAE电泳缓冲液(50×) 250ml
TAE电泳缓冲液(50×) 500ml
TBE电泳缓冲液(5×) 250ml
TAE电泳缓冲液RNA专用(50×) 100ml
蛋白标准(5mg/ml BSA) 1ml
PMSF(100mM) 1ml
5×tris-甘氨酸电泳缓冲液 250ml
SDS-PAGE电泳液(10X) 500ml
SDS-PAGE蛋白上样缓冲液(5X) 2ml
非变性PAGE蛋白上样缓冲液(5X) 2ml
10%过硫酸铵 1.5ml
30% 丙烯酰胺 (29:1) 100ml
40% 丙烯酰胺 (39:1) 100ml
0.5M DTT 2ml
0.5M DTT 10ml
10% SDS(SDS-PAGE电泳) 100ml
TEMED 5ml
1M Tris-HCl,pH6.8 50ml
1.5M Tris-HCl,pH8.8 100ml
Western转膜缓冲液 (20x) 100ml
Western一/二抗稀释液 100ml
Western封闭液(BSA型) 100ml
脱脂奶粉 100g
Western洗涤液 250ml
Western一抗二抗去除液 250ml
BCIP工作液 50mg/ml 100ul
NBT工作液 50mg/ml 100ul
10% Sodium Azide (叠氮钠) 100ml
胰酶细胞消化液 100ml
L-Glutamine(100X) 100ml
Hanks' Balanced Salt Solution 500ml
7.5% NaHCO3溶液 100ml
LB (premixed LB,粉末可配500ml/瓶) 1瓶
青霉素-链霉素溶液(100X) 100ml
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文献和实验Blocking cDNA arrays printed on amine slides
'ok'). ____9. Lift slide rack out of dish, pour in the blocking solution and quickly add slides. (Note: Slides should be lowered into solution quickly to reduce chance of smearing. Thought is that lowering slowly means DNA
Targeted Exon Sequencing by In‐Solution Hybrid Selection
a protocol for the targeted enrichment of exons from randomly sheared genomic DNA libraries using an in?solution hybrid selection approach for sequencing on an Illumina Genome Analyzer II. The steps for designing and ordering a hybrid selection oligo pool
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organism from which you buy secondary antibodies, Jackson Immunolabs
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