- 询价
- Cell Signaling Technology
- 4848
- USA
- 2026年05月28日
- W, IP
- H,M,R,Hm,Mk,B
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- 文献和实验
- 技术资料
- 抗体英文名：
N-WASP (30D10) Rabbit mAb
- 抗原：
synthetic peptide corresponding the sequence of human N-WASP
- 应用范围：
W, IP
- 适应物种：
H,M,R,Hm,Mk,B
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Hm Mk B | Endogenous | 65 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | N-WASP (30D10) Rabbit mAb recognizes endogenous levels of total N-WASP protein. The antibody does not cross-react wth the hematopietic protein, WASP. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding the sequence of human N-WASP. Western Blotting
Western blot analysis of extracts from various cell types, using N-WASP (30D10) Rabbit mAb. IP
Immunoprecipitation of N-WASP from MCF-7 cells, using N-WASP (30D10) Rabbit mAb. Western blot was performed using the same antibody. |
| Background | Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
优化的GlycoWorks HILIC SPE流程用于mAb型样品中N-糖的定量与稳定回收
相关专题 沃特世公司(美国马萨诸塞州米尔福德市) 应用优势： ■GlycoWorks HILIC μElution提取板提供了一种高效的多聚糖纯化装置 ■采用优化的SPE条件定量 并一致性地回收不同范围内的N-糖 ■优化的SPE条件具有出色的方法稳定性 ■可采用2-AB标记的多聚糖标准品确保样品制备和分析 方法的性能 沃特世解决方案：
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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