- 询价
- Cell Signaling Technology
- 4760
- USA
- 2025年11月03日
- IHC-P, IF-F
- R
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Nestin (Rat-401) Mouse mAb
- 抗原：
E15 rat spinal cord
- 应用范围：
IHC-P, IF-F
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
R
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen)
Reactivity Key: R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IHC-P IF-F | R | Endogenous | 207 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Nestin (Rat-401) Mouse mAb detects endogenous levels of nestin protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded rat brain, P1 (left) or P14 (right), using Nestin (Rat-401) Mouse mAb (top) or Neurofilament-L (C28E10) Rabbit mAb #2837 (lower). Note decreased Nestin staining as NFL staining increases in the developing rat brain. IF-F
Confocal immunofluorescent analysis of P1 rat brain (left) and adult rat brain (right) using Nestin (Rat-401) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and is generally accepted as a marker for neural cancer stem cells (3). Nestin expression has alos been observed in astrocytes, retina, cardiac muscle, pancreas and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Fusion of Mouse, Rat, or Hamster Cell
Materials DMEM, high glucose (Life Technologies, Inc. #10313-021 or equivalent) Fetal Bovine Serum (Life Technologies, Inc. #16000-044 or equivalent) L-
Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
技术资料暂无技术资料 索取技术资料
