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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
干冰运输,-80℃保存
- 保质期:
6个月
- 英文名:
Origami (DE3) Competent Cell
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
0.1 mL×10/盒
产品及特点
Origami (DE3)是常规表达宿主菌,用于一般含二硫键蛋白的表达。该菌细胞质二硫化物还原途径有两个突变,增加E. coli细胞质中二硫键的形成。抗性为四环素(12.5 μg/ml)或卡那霉素 (15 μg/ml) 。
其基因型如下:
Δ(ara –leu )7697 Δlac X74 Δpho A Pvu IIpho R ara D139 ahp C gal E gal K rps LF[lac +lac Iq pro ] (DE3) gor 522::Tn10gor B (KanR, TetR)
Origami (DE3)感受态细胞-Origami (DE3) Competent Cell干冰运输,-80℃保存,有效期6个月。R6629-02 Plant RNA Maxi Kit(20)
真菌RNA小量提取试剂盒:从小于100mg真菌组织或真菌细胞中提取总RNA
R6840-00 Fungal RNA Kit(5)
R6840-01 Fungal RNA Kit(50)
R6840-02 Fungal RNA Kit(200)
RNA保护液:保护组织或细胞在12个月内中总RNA不发生降解
R0424-01 RNAsafer Stabilizer Reagent(50ml)
R0424-02 RNAsafer Stabilizer Reagent(250ml)
R5465-01 RNAsafer II Stabilizer Reagent(50ml)
R5465-02 RNAsafer II Stabilizer Reagent(100ml)
SQ 总RNA提取试剂盒
R3053-00 1x108 Cells, 0.5g Tissue
R3053-05 1x109 Cells, 5g Tissue
R3053-40 6.7 x109 Cells, 40g Tissue
一步法RNA提取试剂盒:用传统三相分离法从动物组织/培养细胞中纯化总RNA
R6830-01 RNA-Solv Reagent (100ml)
R6830-02 RNA-Solv Reagent (200ml)
总RNA提取试剂盒:从1X107个真核细胞,100mg动物/植物组织中提取100ug总RNA
R6934-01 Total RNA Kit II(50)
R6934-02 Total RNA Kit II(200)
总RNA中/大量提取试剂盒
R6754-00 Ultra-Pure TotalRNA Midi Kit(2)
R6754-01 Ultra-Pure TotalRNA Midi Kit(10)
R6754-02 Ultra-Pure TotalRNA Midi Kit(25)
R6755-00 Ultra-Pure TotalRNA Maxi Kit(2)
R6755-01 Ultra-Pure TotalRNA Maxi Kit(5)
R6755-02 Ultra-Pure TotalRNA Maxi Kit(20)
总DNA/RNA共提取试剂盒
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文献和实验DNA Origami: Synthesis and Self‐Assembly
., Su, F., Liu, Y., Youngbull, C., Johnson, R., Lindsay, S., Yan, H., and Meldrum, D. 2011. Stability of DNA origami nanoarrays in cell lysate. Nano Lett. 11:1477‐1482. Pal, S
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
Preparation of Competent Cells 制备感受态细胞
competent cell aliquots to-80 degrees C.After the competent cells have been stored for 24 hours check the efficiency of transformation: Use 1 ng 10 ng and 100 ng of any ampicillin resistant plasmid on LBM Amp plates as per transformation protocol for intact
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