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上海玉博生物科技有限公司
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文献和实验Buffer TfbI, pH5.8 500ml 1.47g KOAC 4.95g MnCl2 3.73g KCl 0.74g CaCl2
Transformation Ultra-Competent E. coli
Transformation "Ultra-Competent" E. coli (Inoue Method) Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96 :23-28. Citation Abstract Procedure
Purification of dnEBNA-1/Soft from E. coli BL21 LysS
Inoculate 2ml of 5ml o/n culture of either p3133 (empty vector pET11a)or p3134 (dnEBNA-1/Soft)in E.coli BL21 LysS per 0.5L LB ampicillin (grow two 0.5L cultures of each) Incubate ~2hrs 37℃250rpm until OD600 = 0.4-0.6 Induce with 5ml 100mM ITPG
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