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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20度
- 保质期:
12个月
- 库存:
100
- 供应商:
柯雷生物
- 规格:
50ug
[ PROPERTIES ]
Residues: Cys27~Pro246 (Accession # Q9XSJ0), with N-terminal His-Tag. Host: E. coli Subcellular Location: Nucleus, Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. Predicted isoelectric point: 5.4 Predicted
Molecular Mass: 26.6kDa
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF- CRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDAKTAAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEP
Residues: Cys27~Pro246 (Accession # Q9XSJ0), with N-terminal His-Tag. Host: E. coli Subcellular Location: Nucleus, Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl. Predicted isoelectric point: 5.4 Predicted
Molecular Mass: 26.6kDa
Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSGSEF- CRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDAKTAAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEP
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文献和实验相关实验
了一种诱导巨噬细胞焦亡的结核蛋白 EST12。EST12 结合巨噬细胞中活化的 C 激酶 1(RACK1)的受体,EST12-RACK1 复合体募集去泛素化酶 UCHL5 来促进 NLRP3 的 K48 连接去泛素化,随后导致 NLRP3–caspase-1/11–GSDMD–IL-1β免疫过程。EST12 晶体结构分析表明,氨基酸 Y80 是 RACK1 的关键结合位点。EST12 敲除菌株在体内或体外 MTB 感染中都显示出了高易感性。该研究首次证明了 RACK1 作为病原体的内源宿主感应蛋白
技术资料暂无技术资料 索取技术资料
泛素羧基端酯酶L5(UCHL5)重组蛋白
¥3000




